invitrogen公司A11034抗体现货|赛默飞|thermofisher
Goatanti-RabbitIgG(H+L)HighlyCross-AdsorbedSecondaryAntibody,AlexaFluor488
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QQ：3507623898、3464842061
ProductDetails
TestedApplications
Dilution
FlowCytometry(Flow)
1-10µg/mL
Immunocytochemistry(ICC)
1-10µg/mL
Immunofluorescence(IF)
1-10µg/mL
PublishedApplications
Immunohistochemistry(IHC)
See15publicationsbelow
Immunocytochemistry(ICC)
See22publicationsbelow
Immunohistochemistry(Frozen)(IHC(F))
See10publicationsbelow
Immunohistochemistry-FreeFloating(IHC(Free))
See2publicationsbelow
Immunohistochemistry(Paraffin)(IHC(P))
See2publicationsbelow
MiscellaneousPubMed(MISC)
See468publicationsbelow
ProductSpecifications
SpeciesReactivity
Rabbit
Host/Isotype
Goat/IgG
Class
Polyclonal
Type
SecondaryAntibody
Immunogen
GammaImmunoglobinsHeavyandLightchains
Conjugate
AlexaFluor®488
Excitation/EmissionProfile
Viewspectra 
Form
liquid
Concentration
2mg/mL
Purification
purified
Storagebuffer
PBS,pH7.5
Contains
5mMsodiumazide
Storageconditions
4°C,storeindark
RRID
AB_2576217
Target
IgG
CrossAdsorption
AgainstbovineIgG,goatIgG,mouseIgG,ratIgGandhumanIgG
AntibodyForm
WholeAntibody
ProductSpecificInformation
Tominimizecross-reactivity,thegoatanti-rabbitIgGwholeantibodieshavebeenhighlycross-adsorbedagainstbovineIgG,goatIgG,mouseIgG,ratIgG,andhumanIgG.Cross-adsorptionorpre-adsorptionisapurificationsteptoincreasespecificityoftheantibodyresultinginhighersensitivityandlessbackgroundstaining.Thesecondaryantibodysolutionispassedthroughacolumnmatrixcontainingimmobilizedserumproteinsfrompotentiallycross-reactivespecies.Onlythenonspecific-bindingsecondaryantibodiesarecapturedinthecolumn,andthehighlyspecificsecondariesflowthrough.Furtherpassagesthroughadditionalcolumnsresultin'highlycross-adsorbed'preparationsofsecondaryantibody.Thebenefitsoftheseextrastepsareapparentinmultiplexing/multicolor-stainingexperimentswherethereispotentialcross-reactivitywithotherprimaryantibodiesorintissue/cellfluorescentstainingexperimentswheretheremaybethepresenceofendogenousimmunoglobulins.
AlexaFluordyesareamongthemosttrustedfluorescentdyesavailabletoday.Invitrogen™AlexaFluor488dyeisabright,green-fluorescentdyewithexcitationideallysuitedtothe488nmlaserline.Forstablesignalgenerationinimagingandflowcytometry,AlexaFluor488dyeispH-insensitiveoverawidemolarrange.Probeswithhighfluorescencequantumyieldandhighphotostabilityallowdetectionoflow-abundancebiologicalstructureswithgreatsensitivity.AlexaFluor488dyemoleculescanbeattachedtoproteinsathighmolarratioswithoutsignificantself-quenching,enablingbrighterconjugatesandmoresensitivedetection.Thedegreeoflabelingforeachconjugateistypically2-8fluorophoremoleculesperIgGmolecule;theexactdegreeoflabelingisindicatedonthecertificateofanalysisforeachproductlot.
Usingconjugatesolutions:Centrifugetheproteinconjugatesolutionbrieflyinamicrocentrifugebeforeuse;addonlythesupernatanttotheexperiment.Thisstepwillhelpeliminateanyproteinaggregatesthatmayhaveformedduringstorage,therebyreducingnonspecificbackgroundstaining.Becausestainingprotocolsvarywithapplication,theappropriatedilutionofantibodyshouldbedeterminedempirically.Forthefluorophore-labeledantibodiesafinalconcentrationof1-10µg/mLshouldbesatisfactoryformostimmunohistochemistryandflowcytometryapplications.
Background/TargetInformation
WeofferanextensivelineofInvitrogen™secondaryantibodyconjugateswithwell-characterizedspecificityandlabeledwithawideselectionofpremiumfluorescentdyes,includingInvitrogen™AlexaFluor™fluorescentdyes.Fluorescentsecondaryantibodyconjugatesareusefulinthedetection,sorting,orpurificationofitsspecifiedtargetandidealforfluorescencemicroscopyandconfocallaserscanningmicroscopy,flowcytometry,andfluorescentwesterndetection.Thebreadthoffluorescent Markersweofferallowsourreagentstobetailoredtoalmostanyfluorescentdetectionsystem.
Secondaryantibodiesmaybeprovidedinthreeformats:wholeIgG,divalentF(ab')2fragments,andmonovalentFabfragments.Becauseofthehighdegreeofconservationinthestructureofmanyimmunoglobulindomains,mostclass-specificsecondaryantibodiesmustbeaffinity-purifiedandcross-adsorbedtoachieveminimalcross-reactionwithotherimmunoglobulins.
Oursecondaryantibodyconjugatesaremostcommonlypreparedbyimmunizingthehostanimalwithapooledpopulationofimmunoglobulinsfromthetargetspeciesandcanbefurtherpurifiedandmodified(e.g.,immunoaffinitychromatography,antibodyfragmentation,labelconjugation,etc.)togeneratehighlyspecificreagents.Inthefirstroundofpurification,wholeimmunoglobulinsbindingtotheimmunizingantibodyarerecoveredandmainlyconsistofthe~150-kDaIgGclass.Furtherpurification,forexample,withProteinAorG,removesallunwantedimmunoglobulinclassesexcepttheaffinity-purifiedantibodiesthatreactwiththetarget-specificimmunoglobulinheavyand/orlightchains.
ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.
