Invitrogen公司A21207抗体现货|赛默飞|Thermofisher
Donkeyanti-RabbitIgG(H+L)HighlyCross-AdsorbedSecondaryAntibody,AlexaFluor594
Invitrogen公司A11032抗体现货
手机：18915418616
QQ：3507623898、3464842061
ProductDetails
TestedApplications
Dilution
FlowCytometry(Flow)
1-10µg/mL
Immunocytochemistry(ICC)
2µg/mL
Immunofluorescence(IF)
2µg/mL
Immunohistochemistry(IHC)
1-10µg/mL
PublishedApplications
Immunohistochemistry(IHC)
See6publicationsbelow
Immunohistochemistry(Paraffin)(IHC(P))
See6publicationsbelow
Immunohistochemistry(Frozen)(IHC(F))
See5publicationsbelow
Immunocytochemistry(ICC)
See2publicationsbelow
Immunohistochemistry-FreeFloating(IHC(Free))
See1publicationbelow
MiscellaneousPubMed(MISC)
See13publicationsbelow
ProductSpecifications
SpeciesReactivity
Rabbit
Host/Isotype
Donkey/IgG
Class
Polyclonal
Type
SecondaryAntibody
Immunogen
GammaImmunoglobinsHeavyandLightchains
Conjugate
AlexaFluor®594
Excitation/EmissionProfile
Viewspectra 
Form
liquid
Concentration
2mg/mL
Purification
purified
Storagebuffer
PBS,pH7.5
Contains
5mMsodiumazide
Storageconditions
4°C,storeindark
RRID
AB_141637
Target
IgG
AntibodyForm
WholeAntibody
ProductSpecificInformation
Tominimizecross-reactivity,thesedonkeyanti-rabbitIgGwholeantibodieshavebeenaffinity-purifiedandshowminimumcross-reactivitytobovine,chicken,goat,guineapig,hamster,horse,human,mouse,rat,andsheepserumproteins.Cross-adsorptionorpre-adsorptionisapurificationsteptoincreasespecificityoftheantibodyresultinginhighersensitivityandlessbackgroundstaining.Thesecondaryantibodysolutionispassedthroughacolumnmatrixcontainingimmobilizedserumproteins frompotentiallycross-reactivespecies.Onlythenonspecific-bindingsecondaryantibodiesarecapturedinthecolumn,andthehighlyspecificsecondariesflowthrough.Thebenefitsofthisextrastepareapparentinmultiplexing/multicolor-stainingexperiments(e.g.,flowcytometry)wherethereispotentialcross-reactivitywithotherprimaryantibodiesorintissue/cellfluorescentstainingexperimentswheretheremaybethepresenceofendogenousimmunoglobulins.
AlexaFluordyesareamongthemosttrustedfluorescentdyesavailabletoday.Invitrogen™AlexaFluor594dyeisabright,red-fluorescentdyewithexcitationideallysuitedtothe594nmlaserline.Forstablesignalgenerationinimagingandflowcytometry,AlexaFluor594dyeispH-insensitiveoverawidemolarrange.ProbeswithhighfluorescencequantumyieldandhighphotostABIlityallowdetectionoflow-abundanceBIOLOGicalstructureswithgreatsensitivity.AlexaFluor594dyemoleculescanbeattachedtoproteinsathighmolarratioswithoutsignificantself-quenching,enablingbrighterconjugatesandmoresensitivedetection.Thedegreeoflabelingforeachconjugateistypically2-8fluorophoremoleculesperIgGmolecule;theexactdegreeoflabelingisindicatedonthecertificateofanalysisforeachproductlot.
Usingconjugatesolutions:Centrifugetheproteinconjugatesolutionbrieflyinamicrocentrifugebeforeuse;addonlythesupernatanttotheexperiment.Thisstepwillhelpeliminateanyproteinaggregatesthatmayhaveformedduringstorage,therebyreducingnonspecificbackgroundstaining.Becausestainingprotocolsvarywithapplication,theappropriatedilutionofantibodyshouldbedeterminedempirically.Forthefluorophore-labeledantibodiesafinalconcentrationof1-10µg/mLshouldbesatisfactoryformostimmunohistochemistryandflowcytometryapplications.
Background/TargetInformation
WeofferanextensivelineofInvitrogen™secondaryantibodyconjugateswithwell-characterizedspecificityandlabeledwithawideselectionofpremiumfluorescentdyes,includingInvitrogen™AlexaFluor™fluorescentdyes.Fluorescentsecondaryantibodyconjugatesareusefulinthedetection,sorting,orpurificationofitsspecifiedtargetandidealforfluorescencemicroscopyandconfocallaserscanningmicroscopy,flowcytometry,andfluorescentwesterndetection.Thebreadthoffluorescent Markersweofferallowsourreagentstobetailoredtoalmostanyfluorescentdetectionsystem.
Secondaryantibodiesmaybeprovidedinthreeformats:wholeIgG,divalentF(ab')2fragments,andmonovalentFabfragments.Becauseofthehighdegreeofconservationinthestructureofmanyimmunoglobulindomains,mostclass-specificsecondaryantibodiesmustbeaffinity-purifiedandcross-adsorbedtoachieveminimalcross-reactionwithotherimmunoglobulins.
Oursecondaryantibodyconjugatesaremostcommonlypreparedbyimmunizingthehostanimalwithapooledpopulationofimmunoglobulinsfromthetargetspeciesandcanbefurtherpurifiedandmodified(e.g.,immunoaffinitychromatography,antibodyfragmentation,labelconjugation,etc.)togeneratehighlyspecificreagents.Inthefirstroundofpurification,wholeimmunoglobulinsbindingtotheimmunizingantibodyarerecoveredandmainlyconsistofthe~150-kDaIgGclass.Furtherpurification,forexample,withProteinAorG,removesallunwantedimmunoglobulinclassesexcepttheaffinity-purifiedantibodiesthatreactwiththetarget-specificimmunoglobulinheavyand/orlightchains.
ForResearchUseOnly.Notforuseindiagnosticprocedures.Notforresalewithoutexpressauthorization.
