Signagen品牌货号SL100489介绍|代理商整理
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signagen品牌货号SL100489介绍|代理商整理
signagen品牌介绍
美国SignaGen公司由美国国立卫生研究院的资深科学家创立,是一家新兴的生物试剂生产制造商,主要专注于各种转染试剂的研究与开发,现已成功开发出三大系列DNA/RNA转染试剂。GenJet™体外DNA转染试剂,由于极高的转染效率和极低的毒性迅速赢得科研工作者的青睐。随后推出的GenJet™versionⅡ及GenJet™Plus等具有更高转染效率的升级产品。其中,PolyJet™DNA体外转染试剂采用新型的可生物降解多价阳离子聚合物,进入细胞后该聚合物迅速降解,确保了极低的细胞毒性。目前SignaGen公司已成功地开发出三大系列分别用于体内和体外的DNA和RNA转染试剂,是目前世界上唯一拥有从多聚阳离子聚合物到阳离子型脂质体转染的生物公司。
signagen品牌SL100489介绍
Description
GenJet™ InVitro DNATransfectionReagent(Ver.II)isupgradedversionofGenJet™InVitroDNATransfectionReagent.Adoptinganewchemistry,moreDNAcondensinggroupswereliberatedinGenJet™(Ver.II)(100%vs.80%inoldversion),leADIngto3~4foldsincreaseoftransfectionefficiencywithoutsacrificingcellviABIlity.GenJet™(Ver.II)reagent,1.0ml,issufficientfor~667transfectionsin24wellplatesor~333transfectionsin6wellplates.
FeaturescomparedwitholdversionGenJet™InVitroDNATrasnfectionReagent
-Cell-dependent3~4timeshigherefficiency
-Topchoiceforhard-to-transfectcells
-Efficiencyboostedinthepresenceofserumandantibioticsformostofcelltypes
-Exceptionalhighlevelsofrecombinantproteinproduction
StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.
ComparisonsofTransfectionEfficiencyandCytotoxicityofGenJet™(Ver.II)withOldVersionGenJet™andOtherLeadingProducts
Comparisonoftransfectionefficiency(leftpanel)andtoxicity(rightpanel)GenJet™Ver.IIwitholdversionGenJet™oncommonlyusedmammaliancells. phRL-CMVencodingRenillaLuciferasewasintroducedintocellswiththepresenceofserumandantibiotics.TheRenilliaLuciferaseactivitywasdeterminedwithRenillaLuciferaaseAssaySystem(Promega).Forcytotoxicity,MTTassaywasemployedtodeterminecellviabilitywithuntransfectedcontrolas100%.
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