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Kerafast位于美国波士顿，主要使命是为世界范围内的客户提供特殊的研究用产品。这些产品包括细胞系、抗体、小分子、染料和其他，这些特殊产品的特殊之处在于不能被轻易地买到。自2011年成立以来，世界范围内150余家研究机构向Kerafast提供了他们创新性的试剂产品，使得广大客户在不需要Material Transfer Agreement的情况下，就能比较容易地获取这些产品。
美国Kerafast品牌EQ0001抗体说明书
Anti-Puromycin[3RH11]Antibody
Thismonoclonalantibodytopuromycinprovidesanon-rADIoactivemethodtomeasureratesofglobalproteinsynthesis(mRNAtranslation)incellsortissueslicesincubatedwithpuromycin,oranimalstreatedwithpuromycin invivo.
Highlights:
Allowsforthesimpleevaluationandquantificationoftranslationdirectlyusingstandardimmunochemicalmethods
Advantageousalternativetotraditionalpulse-chasemethods,whichrelyonradioactiveaminoacidlabeling
Puromycinisanaminonucleosideantibiotic,derivedfromthe Streptomycesalboniger bacterium,thatcausesprematurechainterminationduringtranslationtakingplaceintheribosome.Partofthemoleculeresemblesthe3'endoftheaminoacylatedtRNA,makingitusefulforproteintranslationanalysis.
Classicalpulse-chaseorfloodingdosemethodsusedtomonitorproteinsynthesisrelyonthemeasurementofradioactivemethionineandcysteinelabels.Analysisusingpuromycinimmunodetectionisanadvantageousalternativetoradioactiveaminoacidlabeling,andallowsfortheevaluation/quantificationoftranslationdirectlyusingstandardimmunochemicalmethods.
Fromthelaboratoryof ScotR.Kimball,PhD,PennStateCollegeofMedicine
Readrelatedblogpost, PuromycinIncorporationasaMeasureofGlobalProteinSynthesis»
订货信息
| CATALOGNUMBER | PRODUCT | SIZE | AVAILABILITY | |
|---|---|---|---|---|
EQ0001 | Anti-Puromycin[3RH11]Antibody,100ug | 100ug | Instock | |
EQ0005 | Anti-Puromycin[3RH11]Antibody,500ug | 5x100ug | Instock |
SPECIFICATIONS
| ProductType: | Antibody |
| Name: | Anti-Puromycin(3RH11) |
| Host: | Mouse |
| Isotype: | IgG1kappa |
| Clonality: | Monoclonal |
| CloneName: | 3RH11 |
| Specificity: | Thisantibodyrecognizespuromycin. |
| Immunogen: | puromycinhydrochloride |
| Format: | Liquid |
| Purity: | ProteinGpurified |
| Buffer: | PBS |
| TestedApplications: | Westernblotting(1:1,000),ELISAandImmunofluorescencemicroscopy. |
| Storage: | +4C(short-term),-20C(long-term);Avoidrepeatedfreeze/thawcycles. |
| Shipped: | Coldpacks |
