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GenWay/Mouse IGF-1 ELISA Kit/GWB-SKR077/96 Tests

来者：苏州蚂蚁淘

Description:

Short Description: This product is a Sandwich ELISA with a detection method of Colorimetric 450 nm. The Murine IGF-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Murine IGF-1 proteins within the range of 62-6000 pg/ml. The Murine IGF-1 ELISA is capable of recognizing both recombinant and naturally produced Murine IGF-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.• Human IGF-1The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference. • Human beta-NGF, EGF, FGF-8, IGF-BP1, IGF-BP2, IGF-BP3, IGF-BP4, IGF-BP5, IGF-BP7, IGF-2, PDGF-AA, PDGF-AB, PDGF-BB• Murine EGF

Additional Information:

Name

Mouse IGF-1 ELISA Kit

Related Product Names

Insulin-like growth factor I

Gene Symbol

IGF1

Gene Family

ENDOLIG

Gene Fullname

insulin-like growth factor 1

Gene id

16000

Alias Symbols

C730016P09Rik, Igf-1, Igf-I, IGF-I, Insulin-like growth factor I, Somatomedin

Protein Name

Insulin-like growth factor I

Description of Target

This gene encodes a member of the insulin-like growth factor (IGF) family of proteins that promote growth and development during fetal and postnatal life. This gene is predominantly expressed in the liver and the encoded protein undergoes proteolytic proc

Swissprot ID

P05017

Protein Accession Num

NP_001104745.1

Nucleotide Accession Num

NM_001111275.2

Species Reactivity

Mouse

Assay Info

Quantitative Colorimentric Sandwich ELISA

Sensitivity

62 pg/mL

Kit Range

62-6,000 pg/mL

Standard Range

62-6000 pg/ml

Sample Type

Cell lysates, sera and plasma

Product Format

1 x 96-Well Plate or 12 x 8-Well Strips

Specimen/Volumes

Refer to Manual

Swiss Prot Number

P05017

Specificity

The Murine IGF-1 ELISA is capable of recognizing both recombinant and naturally produced Murine IGF-1 proteins. The antigens listed below were tested at 50 ng/ml and exhibited less than 1% cross reactivity.
• Human: IGF-1
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: β-NGF, EGF, FGF-8, IGF-BP1, IGF-BP2, IGF-BP3, IGF-BP4, IGF-BP5, IGF-BP7, IGF-2, PDGF-AA, PDGF-AB, PDGF-BB
• Murine: EGF

Applications

ELISA

GI Number

16000

Format

ELISA Kit. Refer to manual for specific component formats

Stability

6 months

Reconstitution and Storage

4C/6 Months

Reactivity

Mouse

Kit Size

12 x 8-Well Microstrips

Kit Principle

The Aviva Murine IGF-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant mIGF-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Murine IGF-1 cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration

Kit Component

Components	Amount
Capture Antibody Coated Microplate	96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP	12 mL
Ready-to-Use Substrate	12 mL
Stop Solution	12 mL
Wash Buffer (15x)	50 mL
Protein Standard Diluent	12 mL
Sample Diluent	12 mL
Detection Antibody Diluent	12 mL
Biotin-Labeled Detection Antibody	Lyophilized
Protein Standard	Lyophilized (100 ng)