GenWay/Caspase-10 Colorimetric Assay Kit - 100 assays/GWB-AXR078/100 assays
Description:
Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-10 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence AEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate AEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.Summary:
- Detection method- Absorbance (400 or 405 nm)
- Sample type- Cell and tissue lysates
- Species reactivity- Mammalian
- Kit size- Convenient sizes (25, 100, 200, 400 assays)
- Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Kit components:
- Cell Lysis Buffer
- 2X Reaction Buffer
- AEVD-pNA (4 mM)
- DTT (1 M)
- Dilution Buffer
Features and Benefits:
- Simple one-step procedure; takes only 1-2 hours
- Fast and convenient
- Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in AEVD-dependent Caspase activity.
Shipping Conditions: Gel pack
Additional Information:
| Name | Caspase-10 Colorimetric Assay Kit - 100 assays |
|---|---|
| Related Product Names | Caspase-10 Colorimetric Assay Kit, Caspase-10, caspase10, caspase 10, Caspase-10 Substrate, AVED, AEVD-pNA, Caspase-10 assay, Caspase-10 Colorimetric Assay |
| Format | Assay Kit |
| Storage | -20C |
| Intended Use | Research Use Only |
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发布于 ： 2021-09-23
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