GenWay/PDE1C, Active Human Recombinant Protein/GWB-886086/10µg
Description:
Description: Human PDE1C (GenBank Accession No. NM_005020), a.a. 2-634(end), with N-terminal GST-tag, MW=98 kDa, expressed in a Baculovirus-infected Sf9 cell expression system. Specific Activity: 8,180 pmol/min/µg. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 µM cAMP, and 0.1-0.8 ng/μl human PDE1C at 37°C for 20 min. Quantified by 5?-nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. One unit is defined as the amount of enzyme that will convert 1 pmole of 3?, 5?-cAMP to 5? AMP per min at 37 °C. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Stability: >6 months at -80°C. References: 1. Vandeput, F., et al. (2007). J. Biol. Chem. Nov 9;282(45):32749-57. 2. Cygnar, K.D., Zhao, H. (2009). Nat. Neurosci. Apr;12(4):454-62. 3. Ortiz-Capisano, M.C., et al. (2009). Am. J. Physiol. Regul. Integr. Comp. Physiol. Nov;297(5):R1469-76.Host: baculovirus
Tag: N-terminal GST tag
GI Num: NM_005020
MW: 98 kDa
Format: aqueous buffer solution
Formula: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Scientific Category: PDE
Species: human
Additional Information:
| Name | PDE1C, Active Human Recombinant Protein |
|---|---|
| Related Product Names | PDE, phosphodiesterase |
| Molecular Weight | 98 kDa |
| Purity | 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione |
| Source | baculovirus |
| GI Number | NM_005020 |
| Format | Aqueous Buffer Solution |
| Reactivity | Human |
| Storage | -80°C |
| Intended Use | Research Use Only |
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发布于 ： 2021-09-22
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