Description:
qPCRMasterMix(Universal)KAPAhgDNAQuantificationandQCKit
Qualitymatters.
KAPAhgDNAQuantificationandQCKitscontainallthereagentsneededfortheqPCR-basedquantificationandqualityassessmentofhumangenomicDNAsamplespriortoNGSlibraryconstruction.
KitscontainKAPASYBR®FASTqPCRMasterMix,optimizedforhigh-performanceSYBRGreenI-basedqPCR,aswellasapre-dilutedsetofDNAstandardsandprimerpremixestargetingdifferentportionsofahighlyconservedsingle-copyhumanlocus.Absolutequantificationisachievedwiththeprimerpairdefiningtheshortestfragment,whereastheadditionalprimersareusedtoderiveinformationabouttheamountofamplifiabletemplateintheDNAsample.Qualityscores(orQ-ratios)generatedwiththekitmaybeusedtopredicttheoutcomeoflibraryconstruction,ortailorworkflowsforsamplesofvariablequality,particularlyFFPEDNA.
ThemethodiseasytoautomateandcanbeappliedtoanyprocessorworkflowthatrequiresaccuratequantificationofdiluteDNAsamples,orsamplesthatmaycontainahighproportionofDNAthatisrecalcitranttoPCRamplification.
Downloadour KAPAhgDNAQuantificationandQCDataAnalysisTemplate.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Obtainconcentrationandqualityinformationwithasingleassay
- AllowsforaccuratequantificationofdiluteDNAsamples
- QuantificationwithanadditionalprimerpairprovidesaQ-ratiothatisindicativeofsamplequality
Employqualityscores intheanalysisofNGSlibraryconstructionworkflows
- Q-ratioscanprovidevaluableinsightsintothebottlenecksinNGSlibraryconstructionworkflows
- ForFFPEsamples,libraryandsequencequalityisprimarilylimitedbyinefficientconversionofinputDNAtoadapter-ligatedlibrary
- 0.4yieldedlibrariesthatmetminimumsequencequalityrequirements.Dataonfile.">
- 0.4yieldedlibrariesthatmetminimumsequencequalityrequirements.Dataonfile.">
Obtainactionabledataforsamplepreparation
- Q-ratioscorrelatewithkeysequencingmetricssuchasduplicationratesandmeantargetcoverage
- FFPEsampleswithaQscore>0.4yieldlibrariesofacceptablequalitywhenprocessedinstandardsamplepreparationsfortargetcapture
Applications:
Applications- FFPEsamples
- Free-circulatingDNAfromplasmaorserum
- Samplesobtainedbylaser-capturemicrodissectionoffresh,frozenorFFPEtissue
- Forensicsamples
- Cellscollectedbyflowcytometry
- Anyotherlimitingorpreciousclinicalsample
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto12months at-20˚C.
Complete(MasterMix)kitsincludeKAPASYBRFASTqPCRMasterMix(2X),PrimerPremix(41bp,129bpand305bp,10X)andasetof5DNAStandards.PrimerPremixesandDNAStandardsarealsosoldseparately.
Components
Specifications
- SpecDescription
- CompatIBLePlatformAllNGSplatforms
- CompatibleSamplesHumangenomicDNA
- SamplesourcesFFPEtissueCellscollectedbylaser-capturemicrodissectionFlow-sortedcellsFree-circulatingDNAfromplasmaorserumForensicsamplesClinicalsamples
- Standardcurveconcentrationrange2.5ng/µL–10pg/µLor3,080–12copies
- SequencingApplicationsWholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)AmpliconSequencing
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亲子鉴定?
ID,promega.我们实验室主要用的是这两种,由于怕有突变还会备有凯杰的。
如果测序的话,那么就是AB的bigdye吧?
有什么问题我们可以交流。
2 单细胞测序是指单个细胞水平上对基因组进行测序。
3、靶向测序步骤为 样品准备、探针/引物设计、目标序列捕获、文库制备、上机测序、数据分析。
4、单细胞的分离--DNA/RNA的提取和扩增(全基因组扩增和全转录组扩增)---测序以及后续的分析和应用。
相同:使用二代测序
不同:样本处理,文库构建,检测的区域,数据分析流程