Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF Summary
Recombinant Human His6-UBE2N/UBE2V2 Complex is a member of the Ubiquitin conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial His6-UBE2N/UBE2V2 Complex concentration of 0.1-1 μM.
18 kDa (UBE2N), 17 kDa (UBE2V2)
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E2-666
Formulation | 0.88 mg/ml (25 μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM TCEP |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: UBE2N/UBE2V2 Complex
Ubiquitin conjugating Enzyme E2N (UBE2N), also known as Ubiquitin conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive UBE2V2 (human homologue of yeast MMS2) protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively, while human UBE2V2 shares 99% aa sequence identity with its mouse and rat orthologs. The UBE2N/UBE2V2 Complex functions with Ubiquitin ligases (E3s), including RNF111 and RNF8, to synthesize Lys63-linked Ubiquitin chains (2,3) that can either be unanchored or attached to target proteins (4, 5). The UBE2N/UBE2V2 complex has important roles in facilitating responses to various forms of DNA damage (2, 6).
- Hofmann R.M.& Pickart C.M. (1999) Cell 96: 645
- Poulsen, S.L et al. (2013) Journ Cell Biol. 201: 797
- Campbell, S.J. et al. (2012) Journ. Biol. Chem. 287: 23900
- Xia, Z.P. et al. (2009) Nature 461:114.
- Liu, C. et al. (2009) Sci. Signal. 2:ra63.
- Wen, R. et al. (2012) DNA Repair 11: 157
Citation for Recombinant Human His6-UBE2N/UBE2V2 Complex Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- PI5P4K? functions in DTX1-mediated Notch signalingAuthors: L Zheng, SD ConnerProc. Natl. Acad. Sci. U.S.A., 2018;0(0):.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
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但当超声波功率弱的时候,就不是清洗时间长与短的问题了,
而在28KHz到128KHz之间主要用在工序间洗净,40KHz以下的换能器主要用于五金类精密金属类,它强度大,但粗糙,一般精密光学零件都是采用超高频换能器,目前国内的洗净设备还没有达到兆赫兹的制造水平!日本的可以
楼主的问题还不好回答,洗净时间的长短与被洗物及洗净液有关,而并非是越长越好!
只是120KHz的超声机比28KHz的超声机价格高三倍,更高频率的恐怕更是吓人哟。有5分钟的 ,也有半小时的。也和你医疗器械的难易有关,比如含油含血的还是只表面的灰尘的清洗呢!
而如果功率达到一定数值,有时很快便将污垢去除。
若选择功率太大,空化强度将大大增加,清洗效果是提高了,但这时使较精密的零件也产生蚀点,得不偿失,而且清洗缸底部振动板处空化严重,水点腐蚀也增大。
在采用三氯乙烯等有机溶剂时,基本上没有问题,但采用水或水溶性清洗液时,易于受到水点腐蚀,如果振动板表面已受到伤痕,强功率下水底产生空化腐蚀更严重,因此要按实际使用情况选择超声功率。
铭扬超声波清洗机小编为您解答:超声波清洗机的power99%是你机台额定功率的百分比,意思就是机台额定功率是1000W,power99%显示现在机台工作功率达到了990W
2、加速度:液体粒子推动产生的加速度。对于频率较高的超声波清洗机,空化作用就很不显著了,这时的清洗主要靠液体粒子超声作用下的加速度撞击粒子对污物进行超精密清洗。
3、直进流作用:超声波在液体中沿声的传播方向产生流动的现象称为直进流。声波强度在0.5W/cm2时,肉眼能看到直进流,垂直于振动面产生流动,流速约为10cm/s。通过此直进流使被清洗物表面的微油污垢被搅拌,污垢表面的清洗液也产生对流,溶解污物的溶解液与新液混合,使溶解速度加快,对污物的搬运起着很大的作用。
雷士超声波清洗机主要应用于医院手术刀、镊子、止血钳、内镜活检钳、注射用针头、各式大小注射器、试管、玻璃瓶、玻璃片、换药碗、各种盘子、圆桶等放射性、污染性器具的大批量高洁度的清洗和消毒,是医院手术室、化验室、检验室、供应室、牙科、消毒中心以及科研单位,制药厂的必备设备。