Product Name | AnaTagᵀᴹAMCA-X Protein Labeling Kit *Ultra Convenient* |
Size | 1 kit |
Catalog # | AS-72055 |
US$ | $308 |
AMCA-X, SE is one of the most popular blue fluorescent tagging molecules. It is widely used to label antibodies, proteins and small drug molecules. AMCA-X succinimidyl ester contains a seven-atom aminohexanoyl spacer between the fluorophore and the reactive group. This spacer separates the fluorophore from the biomolecule to which it is conjugated, potentially reducing the quenching that typically occurs upon conjugation. AMCA fluorescence can be detected at the emission wavelength of 442±10 nm when excited at 353±10 nm. The AMCA-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all essential components for performing the conjugation reaction and for purifying the AMCA-protein conjugates. The kit is able to perform three conjugation reactions with each reaction labeling up to 5 mg IgG. Kit Size: 3 labeling reactions (3 x 5 mg protein) | |
Detailed Information | DatasheetMaterial Safety Data Sheets (MSDS) |
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例用微物测定孢克罗效价用孢克罗标准品用HPLC或UV测定则用照品;非西丁用作熔点校准物质用熔点标准品测定含量用照品即使同种物质标准品照品规格、标定及用途都能同(北京标准物质网)
我做实验的药物很难买到标准品,但现在有粗品,自己在提纯,想问下各位大牛,在没有标准品的情况下,怎么测提纯后该物质的纯度。谢谢
比如,你的流动相比例、水相pH值、柱温等等条件稍有变化,色谱峰就对不上了。比如,一个碱性物质检测,流动相的pH值稍有波动,这个碱性物质的保留时间就前后波动的很厉害。这个时候就需要定位。
标准品:一般来说,标准品的结构是确定的,含量是确定的。结构就不用说了,保留时间一致就代表是这个东西了。含量,用外标的话就可以计算出样品的含量了。
这个定位品……我倒是没接触过定位品,就理解成用来定位的物质吧。标准品价格比较贵,自制的标准品通过结构确证可以用来定位。这样确保这个位置的色谱峰是这个物质。