Description: | This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples. |
Type: | AssayMax ELISA Kits |
Samples: | Serum Plasma Cell Culture |
Catalog Number: | EA8011-1 |
Species: | Human |
Format: | Sandwich ELISA |
Size: | 96 Well Plate |
Range: | 0.063 - 4 ug/ml |
Research Area: | Cardiovascular, Cancer, Heart Disease |
Entrez Gene: | 341 |
Omim: | 107710 |
UniProt: | P02654 |
Unigene: | Hs.110675 |
Synonyms: | Apolipoprotein C-I, Apo C1, Apo-CI, ApoC-I, Apolipoprotein C1, APOC1, Truncated Apolipoprotein C-I |
Note: | For Research Use Only, Not To Be Used For Diagnostic Purposes |
Product References: | *Xue A et al. (2010) Discovery of serum biomarkers for pancreatic adenocarcinoma using proteomic analysis. Br. J. Cancer 103(3):391-400*Hansen JB et al. (2011) The Apolipoprotein C-I Content of Very-Low-Density Lipoproteins Is Associated with Fasting Triglycerides, Postprandial Lipemia, and Carotid Atherosclerosis. J Lipids. 2011:271062 doi:10.1155/2011/271062*Yan LR et al. (2014) A Pro-Atherogenic HDL Profile in Coronary Heart Disease Patients: An iTRAQ Labelling-Based Proteomic Approach. PLoS One. 9(5):e98368. doi: 10.1371/journal.pone.0098368. eCollection 2014 |
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1.我利用微乳法制备SLN,模型药物、三棕榈酸甘油酯、甘油、泊洛沙姆188,80度下融化,混合均匀,2000rpm搅拌状态下滴加1%PVA水相。冷却至室温时,表面有一层脂质析出。不断增加Lipid:surfacant的比例,当达到1:20时,当冷却至室温时,仍有脂质析出。
新人一枚,想问下各位大侠,有什么好的解决方案不?
我们使用了南京建成的胆固醇,甘油三酯和游离脂肪酸的试剂盒,样本为小鼠肝脏,试剂盒提供的步骤为使用生理盐水或者乙醇作为匀浆介质,3500rpm,10min;
然而我们发现生理盐水作为匀浆介质时,按此离心速度无法获得上清液;若加大离心速度以及延长离心时间,获得的上清液为白色,蛋白浓度检测出来浓度很高,效果十分不理想;若改为乙醇作为匀浆介质时,检测结果甘油三酯很高,游离脂肪酸浓度很低,不知是否涉及脂肪酯化的问题;
希望大家能给我提提建议,给点自己的妙招;或者是否有人提供好用的试剂盒?