SpikeTides™ PTM-Kit 01 - Phospho-Unmodified - quantified

Product Code: SPT-PTM-TQL-K01-Phospho-Unmodified

Collection of absolutely quantified proteotypic peptides containing unmodified phosphopeptides. Amount: 4 peptides (5000 pmol/peptide) Peptide sequences: H-DSTYDLP-R*-Qtag, H-DTDSINLY-K*-Qtag, H-YLSFTPPE-K*-Qtag, H-YNVSTTPYLQSV-K*-Qtag Delivery Format: Polypropylene vials (1 vial per peptide)Application(s): MRM assays, Mass spectrometry, Proteomics, SRM assays, PTM proteomicsIndication(s)/Topic(s): ControlDelivery Time: 2-3 weeks

SpikeTides™ Quantified PTM Reference Kits & PeptidesDespite recent improvements, PTM proteomics is still challenging. Typical problems are low endogenous abundance, low ionization intensity, changed fragmentation, limited stability during proteomics workflows, and/or complex fragmentation spectra interpretation, especially regarding correct PTM site localization. Our defined and carefully selected synthetic PTM reference peptides support PTM proteomics and help to overcome these challenges and are applicable for direct usage as reference material in mass-spectrometry based proteomics.

Available PTMs as peptide kits or individual peptides: Phospho (serine, threonine, tyrosine), Arg(Me, Me2a, Me2s), Lys(Ac, Me, Me2, Me3, Cro, Suc, Mal, Biotin, Glu, For, Prop, But, Hib, GG), Sulfotyrosine, Glyco-Asn(betaDGlcNAc), Glyco-Thr(alphaDGalNAc), Glyco-Ser(alphaDGalNAc). The peptides are part of a collection of 142 peptides that comprises wild-type peptides as well as 23 different post-translational modifications (PTMs).

Quantification with Qtag: Our peptide quantitation technique shows several advantages over traditional quantitation methods. We use a small chemical Qtag attached to the peptide for precise peptide quantitation. The Qtag is released form the peptide during trypsination of the sample. Further information

Benefits of SpikeTides™ Quantified PTM Reference Kits & Peptides - Large range of PTM reference peptides - Selected for high recovery in LC-MS - Stable-isotope labeled (SIL) - Purified and absolutely quantified

SpikeTides™ PTM-Kit 01 - Phospho-Unmodified - quantified

Selected References for SpikeTides™\"Protein Z: A Putative Novel Biomarker for Early Detection of Ovarian Cancer\" Russell et al., Int J Cancer. (2016) - PMID: 26815306 \"RePLiCal: A QconCAT Protein for Retention Time Standardisation in Proteomics Studies\" Holman et al., J Proteome Res. (2016) - PMID: 26775667 \"A Targeted Proteomics Approach for Precision Plant Breeding\" Chawade et al., J Proteome Res. (2015) - PMID: 26704985 \"Quantitative Proteomics of Bronchoalveolar Lavage Fluid in Idiopathic Pulmonary Fibrosis\" Foster et al., J of Proteome Research (2015) - PMID: 25749006 \"Multiple Nutrient Stresses at Intersecting Pacific Ocean Biomes Detected by Protein Biomarkers\" Saito et al., Science (2014) - PMID: 25190794

Testimonials for SpikeTides™\"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPTs various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™_TQL as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets.\"Prof. Andrew Emili, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto\"My group studies the proteomic composition of distinct chromatin domains, the mechanisms that operate to maintain the composition of histone modifications and the associated proteins. For precise and accurate identification and quantification of histone peptides that carry multiple post-translational modifications directly from biological samples JPTs SpikeTides™ _TQL peptide standards proved to be of excellent value for our research in various projects.\"Prof. Dr. Axel Imhof, Adolf-Butenandt Institute, University of Munich, Germany

SpikeTides™ PTM-Kit 01 - Phospho-Unmodified - quantified