Excellgen/Pseudouridine Modified 5 Anti-Reverse Cap Analog (ARCA) capped Cre recombinase mRNA, NLS-C
| Description | Recent studies demonstated that pseudouridine modified mRNA signaficantly reduce PKR activation in cells, thereby enhances translation of the encoded protein after being delivered into culture cells or injected into animals. Pseudouridine modified 5\' Anti-Reverse Cap Analog (ARCA) capped NLS-Cre recombinase (human codon-optimized NLS-Cre mutant) mRNA has been tested for loxP recombination in loxP-RFP-Stop-loxP-GFP reporter cells (e.g., HEK293, Hela, and NIH 3T3), and primary cells. |
Applications | - ex vivo LoxP recombination
- in vivo LoxP recombination
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| Transfection Protocols | 1. Invitrogen RNAiMAX mRNA Transfection Protocol2. Mirus Bio TransIT-mRNA mRNA Transfection Protocol |
| Storage | -20°C or -80°C |
| Shipping | dry ice |
| References | Kariko, K. et al. Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. Mol. Ther. 16, 1833–1840 (2008).Kariko, K., Muramatsu, H., Keller, J.M. & Weissman, D. Increased erythropoiesis in mice injected with submicrogram quantities of pseudouridine-containing mRNA encoding erythropoietin. Mol. Ther. 20, 948–953 (2012).Kormann, M.S. et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. Nat. Biotechnol. 29, 154–157 (2011). Warren, L. et al. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell 7, 618–630 (2010). |
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