reGldA/Recombinant E. coli Glycerol dehydrogenase-蚂蚁淘商城

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reGldA/Recombinant E. coli Glycerol dehydrogenase

2025-11-19

货号：	T-H-03
供应商：	倍诺博生物
英文名：	reGldA
规格：	50ug/100ug/500ug/1mg

DESCRIPTIONSource E. coli-derived, full length (1-367 aa)Accession# P0A9S5SequenceMDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAMLAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENAPVEEIETVAAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQVYAALLVADQYGQRFLQEWEPredicted Molecular Mass 39 kDaSPECIFICATIONSSDS PAGE 39 kDa, reducing conditions.Activity Specific activity: > 25 Units/mgOne unit will oxidize 1.0 µmole of glycerol to dihydroxyacetone per minute at pH 8.0 at room temperature, (note that this is not the optimal pH (~ pH=10), assay performed under pH 8.0 is for the consideration of coupling the assay with other enzymes) Endotoxin Level < 1.0 EU per 1 μg of the protein by the LAL method.Purity > 95%, by SDS-PAGE under reducing conditions and visualized by Coomassie Blue stain at 2 μg per lane.FormulationSupplied as a 0.2 μm filtered solution in 25 mM Tris/HCl, pH 7.5, 300 mM NaCl.PREPARATION AND STORAGEShipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage Avoid repeated freeze-thaw cycles.•6 months from date of receipt, -80 ◦C as supplied.•3 months, -80 ◦C under sterile conditions after opening.ACTIVITY ASSAY PROTOCOLMaterials •Assay Buffer: 100 mM HEPES. (pH 8.0)•Recombinant E. coli glycerol dehydrogenase, reGldA•Glycerol•NAD+, 50 mM stock in deionized water•96-well Clear Plate (BNB, Catalog # 9G-DP33VR-N-LB)•Plate Reader (Model: Tecan Infinite M200 Pro) or equivalentAssay Procedure 1.Dilute reGldA to 10 ng/μL in Assay Buffer.2.Prepare a Substrate Mixture by Diluting NAD+ and glycerol to 10mM and 1 M, respectively, in Assay Buffer.3.Load into a plate 50 μL of enzyme solution and start the reaction by adding 50 μL of Substrate Mix. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of Substrate Mix. Another negative control uses enzyme solution and NAD+ without glycerol.4.Read plate at a wavelength of 340 nm (bottom read) in kinetic mode for 1 minute.5.Calculate specific activity:Specific Activity (μmol/min/mg) = $\"\"$ *Adjusted for Substrate Blank **Using the extinction coefficient 6270 M-1cm-1***Using the path correction 0.32 cm Final assay conditions Per Well:•reGldA: 0.5 μg•NAD+: 5 mM•Glycerol: 500 mMDATA $\"\"$ $\"\"$ BACKGROUND& APPLICATIONGlycerol dehydrogenase (GldA) catalyzes the oxidation of glycerol to dihydroxyacetone using NAD⁺ as electron acceptor or the reverse reaction: the reduction of dihydroxyacetone to glycerol using NADH as the electron donor. This enzyme can be used as a coupling enzyme for the activity assays of various enzymes. It can also be used in the enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD⁺ is catalyzed by the enzyme glycerol dehydrogenase.联系方式：倍诺博生物谷小姐 13632887329/0755-86966133 温馨提示：不可用于临床治疗。

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