YB276Ra013Centromere Protein E (CENPE)Organism Species: Homo sapiens (Human)Instruction manualFOR IN VITRO USE AND RESEARCH USE ONLYNOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES9th Edition (Revised in Jul, 2013)着丝粒蛋白E(CENPE)重组蛋白[ PROPERTIES ]Residues: Ala2450~Arg2590 (Accession # Q02224),with two N-terminal Tags, His-tag and T7-tag. Host: E. coliSubcellular Location: Chromosome, centromere,kinetochore. Cytoplasm, cytoskeleton, spindle.Purity: 95%Endotoxin Level: 1.0EU per 1 g(determined by the LAL method).Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.Predicted isoelectric point: 9.4Predicted Molecular Mass: 20.2kDaApplications: SDS-PAGE; WB; ELISA; IP.(May be suitable for use in other assays to be determined by the end user.)着丝粒蛋白E(CENPE)重组蛋白[ USAGE ]Reconstitute in sterile PBS, pH7.2-pH7.4.[ STORAGE AND STABILITY ]Storage: Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months.Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.着丝粒蛋白E(CENPE)重组蛋白[ SEQUENCES ]The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- A KPYKEEIEDL KMKLVKIDLEKMKNAKEFEK EISATKATVE YQKEVIRLLR ENLRRSQQAQ DTSVISEHTD PQPSNKPLTCGGGSGIVQNT KALILKSEHI RLEKEISKLK QQNEQLIKQK NELLSNNQHL SNEVKTWKER