Anti-p-AR/Androgen Receptor Antibody (156C135.2) | SCBT...
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抗-p-AR/Androgen Receptor 抗体 156C135.2 是小鼠单克隆 IgG,p-AR/Androgen Receptor抗体, 在3篇文献中引用,规格为100 g/mlraised against a synthetic peptide corresponding to amino acids Ser 213/210 of human origin, containing the serine 213 phosphorylation siterecommended for detection of Ser 213 and Ser 210 phosphorylated AR of mouse, rat, human, porcine and canine origin by WB目前,我们还没有完成p-AR/Androgen Receptor 抗体 (156C135.2)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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p-AR Antibody (156C135.2) is a high quality monoclonal p-AR antibody (also designated Androgen Receptor antibody) suitable for the detection of the p-AR protein of mouse, rat, human, porcine and canine origin. p-AR Antibody (156C135.2) is available as the non-conjugated anti-p-AR antibody. Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and reproductive physiology in males. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind active testosterone (T) and dihydrotestosterone (DHT). The rates of association and dissociation of T are about three times more rapid than those of DHT. This difference in binding kinetics may account for the different physiological effects of T and DHT. Androgen binding results in an at least six-fold increase in androgen receptor stability. Akt phosphorylates human AR at Serines 210 and 790. The synthetic androgen R1881 elevates phosphorylation of Serines 308 and 650 in vitro.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Is this antibody tested for porcine AR reactivity in WB? Do you offer any other antibody raised against androgen receptor, estrogen receptor alpha, glucocorticoid receptor or mineralocorticoid receptor that is proved to react with porcine protein?
Thank you for your question. Assay and species recommendations are based on tests with these parameters. Our antibodies against the other receptors you are inquiring about have either not been tested in porcine cells and tissues or are not suitable for that use. Answered by: Tech Support Europe Date published: 2020-11-30
How much sample do you recommend loading on the gel for Western blot detection using p-AR (156C135.2): sc-52894?
We recommend loading 40 ndash;60 micro;g whole cell lysate, 10 ndash;20 micro;g nuclear extract, 5-10 micro;g transfected lysate, or 10 ndash;20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting Answered by: Technical Support Date published: 2017-03-27
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