Biovendor/TNF-α Murine E. coli/RBG20124100/100 μg
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RD1721161000.1 mg产品和服务COVID-19免疫测定下一代测序microRNA和sncRNA蛋白质抗体免疫组织化学内毒素检测和去除产品基于细胞的测定细胞外基质测定DNA/RNA提取仪器/KitsqPCR试剂盒/酶/试剂LAMP TestsBioVendor 分析测试服务- Regulatory status:RUO
- Type:Recombinant
- Source:E. coli
- Other names:Cachectin, Tumor necrosis factor alpha, DIF
- Species:Murine
Type
Recombinant
Description
TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant murine TNF-α is a soluble 156 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Amino Acid Sequence
MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
Source
E. coli
Purity
98%
Biological Activity
The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1×107 units/mg.
Endotoxin
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at-20°C to –80°C.
Storage/Expiration
–20°C
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