货号：	HS-AFM SS-NEX

日本RIBM  HS-AFM超高速视频级原子力显微镜

产品简介：    超高速视频级原子力显微镜（Sample-Scanning High-Speed Atomic Force Microscope ，HS-AFM SS-NEX）是由日本 Kanazawa 大学 Prof. Ando 教授团队研发的，也是世界上第一台可以达到视频级成像的商业化原子力显微镜。HS-AFM突破了传统原子力显微镜\"扫描成像速慢”的限制，能够实现在液体环境下超快速动态成像，分辨率为纳米水平。样品无需特殊固定，不影响生物分子的活性，尤其适用于生物大分子互作动态观测。推出至今，全球已有80多位用户，发表 SCI 文章 200 余篇，包括Science, Nature, Cell 等顶级杂志。

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技术特征：

√ 扫描速度快	√ 探针小，适用于生物样品	√ 自动校正，适合长时间测样
◆ 扫描速度最高可达 20 frame/s◆ 有 4 种扫描台可供选择	◆ 悬臂探针共振频率高，弹簧系数小。避免了对生物样品等的损伤	◆ 悬臂探针可自动漂移校准，适用于长时间观测。

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技术原理：

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应用领域：应用包括：利用 HS-AFM可在纳米尺度动态实时记录生物大分子的运动以及分子间相互作用，包括：     

walking myosin V实时观察	dendrite growth inneuron 实时观察	rotorless F1-ATPase实时观察	light response for D69N实时观察

 

IgG antibody150nm * 150nm	plasmid DNA250nm * 250nm	myosinⅡ500nm * 500nm	bacteriorhodopsin40nm * 40nm
lipid membrane3500nm * 3500nm	350nm poly beads900nm * 900nm	E.coli3000nm * 3000nm	350nm poly beads3000nm * 3000nm

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 应用案例

1.Video imaging of walking myosin V   实时观察myosin V蛋白的运动                                                        N. Kodera et al. Nature 468, 72 (2010). Kanazawa University

2.Real-space and real-time dynamics of CRISPR-Cas9   实时显示CRISPR基因编辑                                      Mikihiro et al. Nature Communications,  (2017). Kanazawa University

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规格参数：              

标准配置
扫描速度  scan speed	50 ms/frame (20 frames/sec)
压电扫描器 piezo range	X: 0.7µm, Y:0.7µm, Z: 0.4µm
样品大小 sample size	1.5mm in diameter
扫描环境 environment	In liquid/In air
控制系统 control system	PID control, Dynamic PID control
significant Function	Scanner active dumping，Drift  correction for cantilever excitation

可选配置
光照装置Light irradiation Unit	Light irradiation unit for the experiments with cagedcompounds.  Variable wavelength: 350-560nm
宽扫描台wide scanner	1frames/s；XY：4µm×4µm, Z：0.7µm
超宽扫描台Amplifiedultra wider scanner	0.1frames/s；XY：30µm×30µm, Z：1.2µm
微流控系统Circulation unit	The observation solutions can be exchanged whilecontinuing AFM observation.

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发表文献（2017年）1. Ando T.; \"Directly watching biomolecules in action by high-speed atomic force microscopy\"; Biophys. Rev. (2017)2. Ando T.; \"High-speed Atomic Force Microscopy for Observing Protein Molecules in Dynamic Action\", Proceedings of SPIE 10328, Selected Papers from the 31st International Congress on High-Speed Imaging and Photonics (2017)3. Aybeke E., Belliot G., Lemaire‐Ewing S., Estienney M., Lacroute Y., Pothier P., Bourillot E., Lesniewska, E.; \"HS‐AFM and SERS Analysis of Murine Norovirus Infection: Involvement of the Lipid Rafts\"; Small 13 1 (2017)4. Cai W, Liu Z., Chen Y., Shang G.; \"A Mini Review of the Key Components used for the Development of High-Speed Atomic Force Microscopy\"; Science of Advanced Materials Vol. 9 Numb. 1 (2017) p.77-885. Colom A., Redondo-Morata L., Chiaruttini N., Roux A., Scheuring S.; \"Dynamic remodeling of the dynamin helix during membrane constriction\"; Proceedings of the National Academy of Sciences 114 21 (2017)6. Dufrêne Y., Ando T., Garcia R., Alsteens D., Martinez-Martin D., Engel A., Gerber Ch., Müller D.; \"Imaging modes of atomic force microscopy for application of molecular and cell biology\"; Nat. Nanotechnol. 12 (2017) p.295-3077. Harada H., Onoda A., Uchihashi T., Watanabe H., Sunagawa N., Samejima M., Igarashi K., Hayashi T.; \"Interdomain flip-flop motion visualized in flavocytochrome cellobiose dehydrogenase using high-speed atomic force microscopy during catalysis\"; Chemical Science (2017)8. Karner A., Nimmervoll B., Plochberger B., Klotzsch E., Horner A., Knyazev D., Kuttner R., Winkler K., Winter L., Siligan Ch., Ollinger N., Pohl P., Preiner J.; \"Tuning membrane protein mobility by confinement into nanodomains\"; Nature Nanotechnology 12 3 (2017) p.260-2669. Keya J., Inoue D., Suzuki Y., Kozai T., Ishikuro D., Kodera N., Uchihashi T., Kabir A., Endo M., Sada K., Kakugo A.; \"High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM\" ; Scientific Reports 7 1 (2017)10. Kim Y.; \"An Advanced Characterization Method for the Elastic Modulus of Nanoscale Thin-Films Using a High-Frequency Micromechanical Resonator\"; Materials 10 7 (2017)11. Kim Y.; \"An evaluation technique for high-frequency dynamic behavior of a sandwich microcantilever beam\"; Journal of Sandwich Structures & Materials (2017)12. Korolkov V., Baldoni M., Watanabe K., Taniguchi T., Besley E., Beton P.; \"Supramolecular heterostructures formed by sequential epitaxial deposition of two-dimensional hydrogen-bonded arrays\"; Nature Chemistry (2017)13. Legrand B., Salvetat J.-P., Walter B., Faucher M., Théron D., Aimé J.-P.; \"Multi-MHz micro-electro-mechanical sensors for atomic force microscopy\"; Ultramicroscopy 175 (2017) p.46-5714. Liao H.-S., Chih-Wen Yang, Hsien-Chen Ko, En-Te Hwu, Ing-Shouh Hwang; \"Imaging initial formation processes of nanobubbles at the graphite–water interface through high-speed atomic force microscopy\"; Applied Surface Science (2017)15. Matsui S., Kureha T., Hiroshige S., Shibata M., Uchihashi T., Suzuki D.; \"Fast Adsorption of Soft Hydrogel Microspheres on Solid Surfaces in Aqueous Solution\"; Angewandte Chemie (2017)16. Mierzwa B., Chiaruttini N., Redondo-Morata L., Moser von Filseck J., König J., Larios J., Poser I., Müller-Reichert T., Scheuring S., Roux A., Gerlich D.; \"Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodeling during cytokinesis\"; Nature Cell Biology (2017)17. Miyata K., Tracey J., Miyazawa K., Haapasilta V., Spijker P., Kawagoe Y., Foster A., Tsukamoto K., Fukuma T.; \"Dissolution Processes at Step Edges of Calcite in Water Investigated by High-Speed Frequency Modulation Atomic Force Microscopy and Simulation\"; Nano Lett. 17 7 (2017) p.4083-408918. Miyazawa K., Watkins M., Shluger A., Fukuma T.; \"Influence of ions on two-dimensional and three-dimensional atomic force microscopy at fluorite–water interfaces\"; Nanotechnology Vol. 28 Numb. 24 (2017)19. Mohamed M., Kobayashi A., Taoka A., Watanabe-Nakayama T., Kikuchi Y., Hazawa M., Minamoto T., Fukumori Y., Kodera N., Uchihashi T., Ando T., Wong R.; \"High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells\"; ACS Nano 11 6 (2017) p.5567-557820. Nievergelt A., Andany S., Adams J., Hannebelle M., Fantner G.; \"Components for high-speed atomic force microscopy optimized for low phase-lag\"; Proceedings of 2017 IEEE International Conference on Advanced Intelligent Mechatronics (AIM) (2017)21. Rangl M., Rima L., Klement J., Miyagi A., Keller S., Scheuring S.; \"Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy\"; Journal of Molecular Biology 429 7 (2017) p.977-98622. Ren J., Zou Q.; \"High-speed dynamic-mode atomic force microscopy imaging of polymers: an adaptive multiloop-mode approach\"; Beilstein J. Nanotechnol. 8 (2017) p.1563-157023. Ricci M., Trewby W., Cafolla C., Voïtchovsky K.; \"Direct observation of the dynamics of single metal ions at the interface with solids in aqueous solutions\"; Scientific Reports 7 43234 (2017)24. Rigato A., Miyagi A., Scheuring S., Rico F.; \"High-frequency microrheology reveals cytoskeleton dynamics in living cells\"; Nature Physics (2017) DOI: 10.1038/NPHYS410425. Ruan Y., Miyagi A., Wang X., Chami M., Boudker O., Scheuring S.; \"Direct visualization of glutamate transporter elevator mechanism by high-speed AFM\"; PNAS 114 7 (2017) p.1584-158826. Sadeghian H., Herfst R., Dekker B., Winters J., Bijnagte T., Rijnbeek R.; \"High-throughput atomic force microscopes operating in parallel\"; Review of Scientific Instruments 88 033703 (2017)27. Sakiyama Y., Panatala R., Lim R.; \"Structural Dynamics of the Nuclear Pore Complex\"; Seminars in Cell and Developmental Biology (2017)28. Shibata M., Watanabe H., Uchihashi T., Ando T., Yasuda R.; \"High-speed atomic force microscopy imaging of live mammalian cells\"; Biophysics and Physicobiology Vol. 14 (2017) p.127-13529. Terahara N., Kodera N., Uchihashi T., Ando T., Namba K., Minamino T.; \"Na+-induced structural transition of MotPS for stator assembly of the Bacillus flagellar motor\"; Science Advances 3 11 eaao4119 (2017)30. Uchihashi T., Scheuring S.; \"Applications of high-speed atomic force microscopy to real-time visualization of dynamic biomolecular processes\"; Biochim Biophys Acta. (2017)31. Usukura E., Narita A., Yagi A., Sakai N., Uekusa Y., Imaoka Y., Ito S., Usukura J.; \"A Cryosectioning Technique for the Observation of Intracellular Structures and Immunocytochemistry of Tissues in Atomic Force Microscopy (AFM)\"; Scientific Reports 7 (2017) 32. Watanabe S., Ando T.; \"High-speed XYZ-nanopositioner for scanning ion conductance microscopy\"; Applied Physics Letters 111 11 (2017)33. Watanabe-Nakayama T., Kodera N., Konno H., Ono K., Teplow D., Yamada M., Ando T.; \"Nano-Space Video Imaging Reveals Structural Dynamics of Fibrous Protein Assembly and Relevant Enzymes\"; Biophysical Journal 112 3 (2017)34. Zhang Y., Tunuguntla R., Choi P., Noy A.; \"Real-time dynamics of carbon nanotube porins in supported lipid membranes visualized by high-speed atomic force microscopy\"; Philosophical Transactions of The Royal Society B Biological Sciences 372 (2017)35. Zhang Y., Yoshida A., Sakai N., Uekusa Y., Kumeta M., Yoshimura S.; \"In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy\" Microscopy 20 (2017) p.272-282 更多文献详见： http://www.highspeedscanning.com/hs-afm-references.html

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