GST-Trap | Chromotek
Description The ChromoTek GST-Trap is a ready to use resin for immunoprecipitation/pulldown of GST fusion proteins. The GST-Trap consists of a GST Nanobody /VHH coupled to agarose beads.
Specificity Glutathione S-Transferase (GST) from Schistosoma japonicum
Applications Immunoprecipitation (IP) / Co-IP Mass spectrometry (MS) On-bead enzyme assays ChIP / RIP analysis
Formats/matrices GST-Trap Agarose GST VHH, recombinant binding protein
Synonyms
GST VHH, anti-GST VHH, GST-Binding-Protein, GST Nanobody, or GST single domain antibody (sdAb)
Effectively immunoprecipitates low expressed GST-fusion proteins Considerably higher affinity than glutathione cellulose and other affinity resins Strong binding even under harsh washing conditions No heavy light chains in your downstream application Dissociation constant KD of 1 nM Compatible to harsh wash conditions: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100 Pulldown of GST-fusion proteins at low concentrations Fully applications validated and characterizedGlutathione S-Transferase (GST) from Schistosoma japonicum is a 220 aa (about 26 kDa) protein, which is commonly used as a protein tag for immunoprecipitation and affinity purification. GST may increase the solubility and expression level of the fusion protein (so-called solubilization tag). Furthermore, GST-tagged fusion proteins are used in protein-protein interaction assays. Applications require highly specific tools to isolate GST-fusion proteins.
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