FormuMax/100nm DOPC/CHOL Liposomes labeled with DiD/F60103F-DD/1 mL
Fluorescent DOPC/CHOL Liposomes labeled with DiD (100nm)
Thefluorescent control liposomescontainthe lipophilic dye DiD incorporated in the bilayers. It is used for tracking the cellular uptake of the liposomes by common methods like confocal, FACS, etc.The far-red fluorescent, lipophilic carbocyanineDiDis a longer-wavelength DiI analog. It is an oil at room temperature and weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments.
Specifications
Lipid composition: DOPC/CHOL (54:45 mol/mol)
Lipid concentration: 50 mM (50-55 mM)
Mean particle size:100 nm (90-120 nm)
Hydration buffer:10% sucrose, 20mM HEPES, pH7.3 ± 0.2
DiD:0.5 mM (0.48mg/mL)
Stability:6 months for unopened vials
Storage: 2-8ºC
DiD;DiIC18(5) oil (1,1-Dioctadecyl-3,3,3,3-Tetramethylindodicarbocyanine Perchlorate)(Invitrogen D-307)
Ex/Em:644/665nm
CAS Number:127274-91-3
DOPC:1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL: cholesterol
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