MBL International\'s Anti-LC3 (Human) pAb is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blot. Description Macroautophagy mediates the bulk degradation of cytoplasmic components. These components are delivered to lysosomes via autophagosomes. The microtubule-associated protein 1 light chain 3 (LC3), a homologue of yeast Atg8 (Aut7/Apg8), localizes to autophagosomal membranes after post-translational modifications. The C-terminal fragment of LC3 is cleaved immediately following synthesis to yield a cytosolic form called LC3-I (18 kDa). A subpopulation of LC3-I is further converted to an autophagosome-associating form, LC3-II (16 kDa). This antibody can detect both forms of LC3 Product SpecsItemAnti-LC3 (Human) pAbCompanyMBL InternationalPriceSupplier PageView Company Product PageCatalog NumberPM036Quantity100 ulApplicationsFCM, IC, IH, IPP, WBConjugate/TagUnconjugatedFormatIn PBS/50% glycerol, pH 7.2ReactivityHumanAntigenLC3IsotypeIgGHostRabbitAntibody TypePrimaryRegulatory StatusRUOImmunogenRecombinant human LC3 (MAP1LC3B :1-120 a.a.)ClonalityPolyclonal References Guo JL, Buist A, Soares A, Callaerts K, Calafate S, Stevenaert F, Daniels JP, Zoll BE, Crowe A, Brunden KR, et al. The Dynamics and Turnover of Tau Aggregates in Cultured Cells: INSIGHTS INTO THERAPIES FOR TAUOPATHIES. J Biol Chem. 2016 Jun 17;291(25):13175-93.Kaini RR, Sillerud LO, Zhaorigetu S, Hu CA. Autophagy regulates lipolysis and cell survival through lipid droplet degradation in androgen-sensitive prostate cancer cells. Prostate. 2012 Sep 15;72(13):1412-22.Sano O, Kazetani K, Funata M, Fukuda Y, Matsui J, Iwata H. Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition. FEBS Lett. 2016 Jun;590(11):1576-85.Seillier M, Peuget S, Gayet O, Gauthier C, N\'Guessan P, Monte M, Carrier A, Iovanna JL, Dusetti NJ. TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death. Cell Death Differ. 2012 Sep;19(9):1525-35 (1) cAMP-mediated autophagy inhibits DNA damage-induced death of leukemia cells independent of p53OncotargetJuly 13, 2018Seham Skah, Nina Richartz, Eva Duthil, Karin M. Gilljam, Christian Bindesb ll, Elin Hallan Naderi, Agnete B. Eriksen, Ellen Ruud, Marta M. Dirdal, Anne Simonsen, Heidi Kiil Blomhoff (2) The E3 ubiquitin ligase NEDD4 enhances killing of membrane-perturbing intracellular bacteria by promoting autophagyAutophagyDecember 17, 2017Gang Pei, Hellen Buijze, Haipeng Liu, Pedro Moura-Alves, Christian Goosmann, Volker Brinkmann, Hiroshi Kawabe, Anca Dorhoi, Stefan H. E. Kaufmann (3) Melatonin enhances sorafenib actions in human hepatocarcinoma cells by inhibiting mTORC1/p70S6K/HIF-1a and hypoxia-mediated mitophagyOncotargetAugust 24, 2017N stor Prieto-Dom nguez, Carolina M ndez-Blanco, Sara Carbajo-Pescador, Flavia Fondevila, Andr s Garc a-Palomo, Javier Gonz lez-Gallego, Jos L. Mauriz (4) Ohmyungsamycins promote antimicrobial responses through autophagy activation via AMP-activated protein kinase pathwayScientific ReportsJune 13, 2017Tae Sung Kim, Yern-Hyerk Shin, Hye-Mi Lee, Jin Kyung Kim, Jin Ho Choe, Ji-Chan Jang, Soohyun Um, Hyo Sun Jin, Masaaki Komatsu, Guang-Ho Cha, Han-Jung Chae, Dong-Chan Oh, Eun-Kyeong Jo (5) Exploiting macrophage autophagy-lysosomal biogenesis as a therapy for atherosclerosisNature CommunicationsJune 7, 2017Ismail Sergin, Trent D. Evans, Xiangyu Zhang, Somashubhra Bhattacharya, Carl J. Stokes, Eric Song, Sahl Ali, Babak Dehestani, Karyn B. Holloway, Paul S. Micevych, Ali Javaheri, Jan R. Crowley, Andrea Ballabio, Joel D. Schilling, Slava Epelman, Conrad C. Weihl, Abhinav Diwan, Daping Fan, Mohamed A. Zayed, Babak Razani (6) UVRAG Deficiency Exacerbates Doxorubicin-Induced CardiotoxicityScientific ReportsFebruary 22, 2017Lin An, Xiao-wen Hu, Shasha Zhang, Xiaowen Hu, Zongpei Song, Amber Naz, Zhenguo Zi, Jian Wu, Can Li, Yunzeng Zou, Lin He, Hongxin Zhu (7) Prenatal alcohol exposure impairs autophagy in neonatal brain cortical microvesselsCell Death amp; DiseaseFebruary 9, 2017Virginie Girault, Vianney Gilard, Florent Marguet, C line Lesueur, Michelle Hauchecorne, Yasmina Ramdani, Annie Laquerri re, St phane Marret, Sylvie J gou, Bruno Jose Gonzalez, Carole Brasse-Lagnel, Soumeya Bekri (8) Glycycoumarin inhibits hepatocyte lipoapoptosis through activation of autophagy and inhibition of ER stress/GSK-3-mediated mitochondrial pathwayScientific ReportsNovember 30, 2016Enxiang Zhang, Shutao Yin, Xinhua Song, Lihong Fan, Hongbo Hu (9) The Aurora kinase A inhibitor TC-A2317 disrupts mitotic progression and inhibits cancer cell proliferationOncotargetOctober 4, 2016Yoo Hong Min, Wootae Kim, Ja-Eun Kim (10) The Dynamics and Turnover of Tau Aggregates in Cultured Cells: INSIGHTS INTO THERAPIES FOR TAUOPATHIES.The Journal of biological chemistryJune 17, 2016Jing L Guo, Arjan Buist, Alberto Soares, Kathleen Callaerts, Sara Calafate, Frederik Stevenaert, Joshua P Daniels, Bryan E Zoll, Alex Crowe, Kurt R Brunden, Diederik Moechars, Virginia M Y Lee (11) Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition.FEBS lettersJune 1, 2016Osamu Sano, Kenichi Kazetani, Masaaki Funata, Yasunori Fukuda, Junji Matsui, Hidehisa Iwata (12) Autophagy regulates lipolysis and cell survival through lipid droplet degradation in androgen-sensitive prostate cancer cells.The ProstateSeptember 15, 2012Ramesh R Kaini, Laurel O Sillerud, Siqin Zhaorigetu, Chien-An A Hu (13) TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death.Cell death and differentiationSeptember 1, 2012M Seillier, S Peuget, O Gayet, C Gauthier, P N Guessan, M Monte, A Carrier, J L Iovanna, N J Dusetti (14) Suppression of basal autophagy reduces lung cancer cell proliferation and enhances caspase-dependent and -independent apoptosis by stimulating ROS formation.AutophagyJuly 1, 2012Vitaliy O Kaminskyy, Tatiana Piskunova, Irina B Zborovskaya, Elena M Tchevkina, Boris Zhivotovsky (15) Beneficial effects of fenofibrate in retinal pigment epithelium by the modulation of stress and survival signaling under diabetic conditions.Journal of cellular physiologyJune 1, 2012Soledad Miranda, gueda Gonz lez-Rodr guez, Marta Garc a-Ram rez, Jes s Revuelta-Cervantes, Cristina Hern ndez, Rafael Sim , ngela M Valverde (16) Effect of persistent activation of phosphoinositide 3-kinase on heart.Life sciencesApril 20, 2012Shinichiro Niizuma, Yasutaka Inuzuka, Junji Okuda, Takao Kato, Tsuneaki Kawashima, Yodo Tamaki, Yoshitaka Iwanaga, Yuki Yoshida, Rie Kosugi, Kayo Watanabe-Maeda, Yoji Machida, Shingo Tsuji, Hiroyuki Aburatani, Tohru Izumi, Toru Kita, Takeshi Kimura, Tetsuo Shioi (17) TGF -activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy.Journal of biochemistryFebruary 1, 2012Giichi Takaesu, Takashi Kobayashi, Akihiko Yoshimura (18) Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle.Cell host microbeJanuary 19, 2012Tregei Starr, Robert Child, Tara D Wehrly, Bryan Hansen, Seungmin Hwang, Carlos L pez-Otin, Herbert W Virgin, Jean Celli (19) Ras-related tumorigenesis is suppressed by BNIP3-mediated autophagy through inhibition of cell proliferation.Neoplasia (New York, N.Y.)December 1, 2011Shan-Ying Wu, Sheng-Hui Lan, Da-En Cheng, Wei-Kai Chen, Cheng-Huang Shen, Ying-Ray Lee, Roberto Zuchini, Hsiao-Sheng Liu (20) Dysfunction of autophagy participates in vacuole formation and cell death in cells replicating hepatitis C virus.Journal of virologyDecember 1, 2011Shuhei Taguwa, Hiroto Kambara, Naonobu Fujita, Takeshi Noda, Tamotsu Yoshimori, Kazuhiko Koike, Kohji Moriishi, Yoshiharu Matsuura Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists.