Sciencell/GeneQuery™ Human AOC3-AOC4P Pseudogene Transcription Analysis qPCR Kit/GK804/1 Ea
This kits are qPCR ready in a 96-well plate format,with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA.
Note: all gene and pseudogene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).
Pseudogenes are DNA segments introduced into the genome by gene duplication or RNA retrotransposition. The majority of pseudogenes bear high sequence similarity to their corresponding functional genes, but have lost some or all of the functionality. ScienCells GeneQuery™ Human ZNF300-ZNF300P1 Pseudogene Transcription Analysis qPCR Kit (GQP-ZNF300P1) is designed to facilitate the transcript quantification of pseudogene ZNF300P1 and its corresponding functional gene, ZNF300. Human ZNF300P1 pseudogene and ZNF300 gene are both located on chromosome 5. Although ZNF300 gene and ZNF300P1 pseudogene share high sequence similarity, GQP-ZNF300P1 guarantees that each primer set only amplifies the designated gene or pseudogene without cross-amplification for precise quantification.
GeneQuery™ pseudogene transcription analysis qPCR kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene or pseudogene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
Specifications:
Catalog No. | GK804 |
Country of Manufacture | United States |
Product Code | GQP-AOC4P |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | For research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |
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