Zyagen/FOXO1 Antibody/9774/1 Ea
Background
FOXO1 Antibody: FOXO1, also known as FKHR or ForkHead in Rhabdomyosarcoma, is a 70 kDa protein which is a ubiquitously expressed member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In insulin-responsive tissues, stress or nutrient abundance triggers phosphorylation by PKB/AKT, blocking nuclear translocation and activity. Genetic mutations involving FOXO1A are a cause of alveolar rhabdomyosarcoma (RMS2). Recent studies link the anti-tumor activity of FOXO1 and the process of autophagy.
Description
![\"FOXO1](\"http://www.prosci-inc.com/image/product/23/26/5811.gif\")
Western blot analysis of FOXO1 in Hela cell lysate with FOXO1 antibody at 1 μg/mL.![\"FOXO1](\"http://www.prosci-inc.com/image/product/23/26/d_1609.jpg\")
Immunocytochemistry of FOXO1 in HeLa cells with FOXO1 antibody at 4 μg/mL.Specifications
Additional Names
FOXO1 Antibody: Forkhead box O1, FKH1, FKHR, Forkhead in rhabdomyosarcoma, FOXO1ADisclaimer
This product is for research use only.User Note
Optimal dilutions for each application to be determined by the researcher.Immunogen
FOXO1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO1.Isotype
IgGClonality/Clone
This is a polyclonal FOXO1 Antibody.Conjugate
NoneFormulation
Physical State: LiquidConcentration: 1 mg/mLPurification
FOXO1 Antibody is affinity chromatography purified via peptide column.Host
FOXO1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.Application
FOXO1 antibody can be used for detection of FOXO1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.Tested Application
E, WB, ICC, IFBuffer
FOXO1 Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat.No. ZP-5811 - FOXO1 PeptideStorage
FOXO1 antibody can be stored at 4ËšC for three months and -20ËšC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Species Reactivity
H, M, RProtein GI Number
9257222Protein Accession Number
NP_002006Reference
- Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99.
- Greer EL and Brunet A. FOXO transcription factors at the interface between longevity and tumor suppression. Oncogene2005; 24:7410-25.
- Brunet A, Bonni A, Zigmond MJ, et al. Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor. Cell1999; 96:857-68.
- Linardic CM. PAX3-FOXO1 fusion gene in rhabdomyosarcoma. Cancer Lett.2008; 270:10-8. ]
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