Agrisera/PRP39a | pre-mRNA-processing factor 39/AS14 2784/
| | product information | | Background | | PRP39a (pre-mRNA-processing factor 39) is involved in RNA processing, mRNA 5-splice site recognition, regulation of timing of transition from vegetative to reproductive phase. | | Immunogen | | Recombinant, full length PRP39a of Arabidopsis thaliana , UniProt: F4I448,TAIR: At1g04080 | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Serum | | Format | | Lyophilized | | Quantity | | 50 µl | | Reconstitution | | For reconstitution add 50 µl of sterile water. | | Storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | Tested applications | | western blot (WB) | | Related products | | AS14 2785 | anti-PRP40B | pre-mRNA-processing protein 40B, rabbit antibodies collection of antibodies to micro RNA Recommended secondary antibody for ECL detection Plant protein extraction buffer Secondary antibodies | | Additional information | | | | application information | | Recommended dilution | | 1: 2000 - 1: 5 000 (WB) | | Expected | apparent MW | | 91 | 100 kDa | | Confirmed reactivity | | Arabidopsis thaliana | | Predicted reactivity | | Brassica rapa | | Not reactive in | | | | Additional information | | | | Selected references | | to be added when available, antibody released in Februrary 2016. | application example![\"western](\"https://www.ebiomall.com/images/agrisera/201709/AS14) 40 µg of total protein from 14-day-old seeldlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 mM Tris HCl, 10 % glycerol, 5 mM EGTA, 0.15 M NaCl, 0.75 % Triton X100, 0.05 % SDS, 1mM DTT, 1x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14 % SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5 % milk in TBS+0.1 % Tween ON at 4ºC with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 (left panel) or 1: 2000 (right panel) in TBS-T (0.1 % Tween) for 1 h at RT with agitation. Blot was incubated in secondary antibody (goat anti-chicken HRP conjugated, AS09 602 Agrisera) in 1: 10 000 diution for 1 h at RT with agitation in TBS 0.2 % Tween. The blot was washed as above and developed for 5 minutes with ECL Prime, GE Healthcare, according to manufacturers instructions. Exposure time was 1 min. Courtesy of M.Sc. Agata Stępień, Department of Gene Expression, Adam Mickiewicz University, Poland
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