Agrisera/Goat anti-Dog IgG (H&L), FITC conjugated/AS16 3126/
| | product information | | Background | | Goat anti-Dog IgG (H&L), FITC conjugated is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays. | | Immunogen | | Purified normal IgG isolated from pooled dog serum | | Host | | Goat | | Clonality | | Polyclonal | | Clone | | | | Purity | | Protein G purified IgG | | Format | | Lyophilized in PBS, pH 7.2 | | Quantity | | 2 ml | | Reconstitution | | For reconstitution please add 2 ml of sterile destilled water. | | Storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in +4°C up to 7 days. | | Tested applications | | ELISA (ELISA), immunocytochemistry (ICC), immunohistochemistry (frozen tissue) (IHC), (in)direct immunofluorescence (IF) | | Related products | | other FITC conjugated secondary antibodies to dog IgG Secondary antibodies | | Additional information | | | |
| application information |
| Recommended dilution | | 1:20 - 1:120 (ICC, IHC and IF)The optimal working dilution should be established by titration by the end user. Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal. |
| Expected | apparent MW | | |
| Confirmed reactivity | | dog IgG |
| Predicted reactivity | | |
| Not reactive in | | This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion. |
| Additional information | | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. |
| Selected references | | to be added when available, antibody released in January 2016. |
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