Agrisera/HRP | Horseradish peroxidase (affinity purified)/AS09 549/
| | product information | | Background | | Horseradish peroxidaseremoves hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress. | | Immunogen | | native peroxidase isolated and purified from horseradish | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified IgG | | Format | | Lyophilized in phosphate buffer saline | | Quantity | | 1 mg | | Reconstitution | | For reconstitution please add 0.5 ml of sterile destilled water. | | Storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | Tested applications | | western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC) | | Related products | | AS09 548| anti-HRP horseradish Plant protein extraction buffer Secondary antibodies | | Additional information | | antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition. | |
| application information |
| Recommended dilution | | 1: 1000 - 1: 10 000 for techniques listed above |
| Expected | apparent MW | | 40 kDa |
| Confirmed reactivity | | Armoracia rusticana |
| Predicted reactivity | | Armoracia rusticana |
| Not reactive in | | no confirmed exceptions from predicted reactivity known in the moment |
| Additional information | | Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran. |
| Selected references | | to be added when available |
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