Agrisera/DEG15 | Endopeptidase (peroxisomal marker)/AS10 711/
| | product information | | Background | | DEG15 is a peroxisomal Deg-protease with endopeptidase activity. This protease cleaves specifically substrates with Cys in the P1 and P2 position and acts as peroxisomal processing peptidase. Alternative names: At1g28320/F3H9_2 | | Immunogen | | synthetic peptide derived from Arabidopsis thaliana DEG15, Q8VZD4 (At1g28320) | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinity purified serum | | Format | | Lyophilized | | Quantity | | 100 µg | | Reconstitution | | For reconstitution add 100 µl of sterile water. | | Storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | Tested applications | | western blot (WB) | | Related products | | AS07 246 | anti-Deg1 | chloroplastic DegP-type serine protease 1 , rabbit antibodies AS10 1581 | anti-Deg2 | chloroplastic DegP-type serine protease 2, rabbit antibodiesAS11 1756 | anti-DegP7 | protease Do-like 7, rabbit antibodiesAS14 2767 | anti-Deg8 | protease Do-like 8, chloroplastic, rabbit antibodiesAS16 3110 | anti-Cp2 | Cysteine protease rabbit antibodies collection of antibodies to chloroplastic proteases Plant protein extraction buffer Secondary antibodies | | Additional information | | | | application information | | Recommended dilution | | 1 : 500 with standard ECL (WB) | | Expected | apparent MW | | 76 kDa | | Confirmed reactivity | | Arabidopsis thaliana, Citrullus vulgaris, Physcomitrella patens | | Predicted reactivity | | dicots including: Solanum lycopersicum, Sorghum vulgare, Ricinus communis, Vitis vinifera, monocots: Oryza sativa, Zea mays, trees: Populus balsamifera, algae: Micromonas sp. | | Not reactive in | | no confirmed exceptions from predicted reactivity known in the moment | | Additional information | | to be added when available | | Selected references | | Schuhmann et al. (2008). The DEG15 serine protease cleaves peroxisomal targeting signal 2-containing proteins in Arabidopsis.Plant Physiol. 4: 1847-1856.Helm et al. (2007). Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants.PNAS 27: 11501-11506. |
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