Agrisera/Sec21p | Gamma subunit, COP vesicles/AS08 327/-蚂蚁淘商城

新闻动态

Agrisera/Sec21p | Gamma subunit, COP vesicles/AS08 327/

2025-05-10


product information
Background		Sec21p is a constituent of the COPI vesicle coatomer.
Immunogen		GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		200 µl
Reconstitution		For reconstitution add 200 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Immunofluorescence (IF), Western blot (WB)
Related products		AS10 683 \| anti-HDEL \| endoplasmic reticulum retention signa, 100 µg AS10 683-25 \| anti-HDEL \| endoplasmic reticulum retention signal, 25 µg AS08 325 \| anti-Arf1 \| ADP-ribosylation factor 1, Golgi marker, rabbit antibody Plant protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1: 1000 (IF), 1: 1000 (WB)
Expected \| apparent MW		98 kDa
Confirmed reactivity		Arabidopsis thaliana, Zea mays
Predicted reactivity		Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Coffea canephora, Eutrema salsugineum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Oryza sativa, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays,
Not reactive in		Nicotiana tabacum, Microsporidia sp.
Additional information		This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot. References describing immunolocalization (IF) and (IG) studies: Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36. Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
Selected references		Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114. Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788. Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Tanaka et al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization). Hopff et al. (2013). The plasma membrane proteome of maize roots grown under low and high iron conditions. J Proteomics Jan 24. Pimpl et al (2000). In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell. 2000 Nov;12(11):2219-36.

application example

50 µg of total protein from (1) Nicotiana tabacum protoplast total protein, (2) Arabidopsis thaliana protoplast soluble protein, (3) Arabidopsis thaliana protoplast total protein were separated on 10 % SDS-PAGE and blotted 2h to nitrocellulose (Semi-dry, 200mA). Filters were blocked over night with 5% low-fat milk powder in TBS and probed with anti-Sec21p antibodies (AS08 327, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP) in TBS-Tween. Signal was detected with standard ECL andexposure time for this image was 1 minute.

Protoplasts were extracted in 50mM Tris, 10 mM EDTA and Triton X100, 0.02%.

$\"Western$

Immunofluorescence

Immunofluorescence labelling of rabbit anti-SEC21 (gamma subunit of COP vesicles; red) in 5-day-old root epidermal cells of Arabidopsis thaliana expressing ER-mGFP5-HDEL (ER marker; green). The antibody was diluted 1:1000 and the secondary antibody, donkey anti-rabbit CY5-coupled (Jackson ImmunoResearch) was diluted 1:300. The nuclei were stained with DAPI (blue).

Courtesy of Dr. Anna Gustavsson and Dr. Markus Grebe

$\"immunofluorescence$

本文链接： https://www.ebiomall.cn/b36-agrisera/info-1548212084.html

免责声明本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容。

没有了

Agrisera/Rhamnogalacturonan-Ib | (CCRC-M61)/AS16 3237/

2020-07-03

Agrisera/PSA | prostate specific antigen/AS10 1352/

2020-07-25

Agrisera/m6A | N6-methyladenosine (polyclonal)/AS17 4121/

2020-08-07

Agrisera/6x Histidine, Biotin Conjugated/AS15 2934/