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nThe extraction of RNA was performed using the RNeasy mini kit n(Qiagen). RT-qPCRs for RNA expression were performed by using nthe KAPA SYBR FAST One-step RT-qPCR kit (KAPA biosystems). nRT-qPCRs were performed on anMx3005p Real Time PCR System n(Stratagene). All PCR products were verified on agarose gels nstained with ethidium bromide. We designed and synthesized nthe following primers: ACE1-sense, 5′-caacatcaccacagagaccagcaa- n3′; ACE1-antisense, 5′-ggtgtctcgtacatagacctccac-3′; reninsense, n5′-caatccgagaaagcctgaag-3′; renin-antisense, 5′-ctgtgtcaccgtgattccac- n3′; GAPDH-sense, 5′-cgagatccctccaaaatcaa-3′; and nGAPDH-antisense, 5′-gtcttctgggtggcagtgat-3′
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