Medchemexpress/3-Methyladenine(Synonyms: 3-MA)/HY-19312/10mM*1mL in DMSO
- 产品编号： HY-19312-50mg
- 美 元 价： $60.00
- 市 场 价： ¥1200.00
- 品 牌： MCE
- 产 地： 美国(厂家直采)
- 公 司： Medchemexpress
- 产品分类： 小分子>生物分子>生化试剂>
- 公司分类：
- 联系 Q Q: 3392242852
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CustomerValidation
- •JDermatolSci.2017Oct11.
- •BiochemBiophysResCommun.2016Dec2;481(1-2):90-96.
| Description |
3-MethyladenineisaselectivePI3KinhibitorwithIC50of25μMand60μMforVps34andPI3Kγ,respectively,anditcaninhibitallPtdIns3Ksat10mM. |
|---|---|
| IC50&Target |
IC50:25μM(Vps34),60μM(PI3Kγ) |
| InVitro |
3-methyladenineshowssomelimitedVps34preferenceinvitro,withanIC50of25µMforVps34ascomparedwith60µMforPtdIns3Kγ,and3-methyladenine(10mM)caninhibitallPtdIns3Ks[1].3-methyladenine(3-MA,5mM)suppressesautophagyinHeLacellsunderbothglucose-freeconditionsandnormalconditions.3-methyladenineinducescaspase-dependentcelldeathinHeLacells,andthedeathoccursindependentlyoftheinhibitionofautophagy.3-MA(1mM)significantlyshortensthedurationofnocodazole-induced-prometaphasearrest[2].Alargenumberofneuronsfromthe3-MAgroupareintheearlystageofapoptosis,displayingcellshrinkage,nuclearandchromatincondensationandaggregationattheedgeofthenucleus[3]. |
| InVivo |
3-methyladenine-treatmentsignificantlyaggravatesneurologicalsymptomsandapoptoticcelldeathafterSAH[3]. |
| References |
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| PreparingStockSolutions |
Pleaserefertothesolubilityinformationtoselecttheappropriatesolvent.
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CellAssay [2] |
CellviABIlityisdeterminedbyatrypanblueexclusionassay.Cellsareculturedinthemediumwith3-Methyladenine.BothadherentandfloatingcellsarecollectedandsUSPendedinphosphatebufferedsaline(PBS,pH7.4)atafinaldensityof1-2×106/mL.Anequalvolumeof0.4%trypanbluesolution(w/v,inPBS)isaddedtothecellsuspensionandmixedthoroughly.Afterincubationatroomtemperaturefor3min,cellcountingisperformedusingahemacytometer.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly. |
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AnimalAdmiNISTration [3] |
3-Methyladenineisdissolvedinnormalsaline. Animalsarepositionedinastereotacticframeandtreatedwith5-μLintracerebralventricularinjectionsofautophagyinducerRAP(20 pmol),inhibitor3-Methyladenine(3-MA,400 nmol)orvehicle20 minbeforeSAHonset.Intracerebralventricularinjectionsaremadeintotheright lateralventricle atcoordinatesof0.8 mmanteriortoposteriorbregma,1.5 mmmidtolateral,3.8 mmdorsaltoventral.Aftertheinjection,thesyringeisleftinthesitefor30 sbeforeremoval,andtheburrholeisfilledwathbonewax.Drugdosesareselectedonthebasisofpreviouspublishedstudiesandpilotexperimentsinourlaboratory.3-MAisdissolvedinnormalsalinebyheatingthesolutionto60-70°Cimmediatelybeforetreatment.RAPisfirstdissolvedinethanolandthendilutedwithnormalsaline(finalethanolconcentration<2%).=""mce=""has=""not=""independently=""confirmed=""the=""accuracy=""of=""these=""methods.=""they=""are=""for=""reference=""> |
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| References |
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| MolecularWeight |
149.15 |
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| Formula |
C₆H₇N₅ |
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| CASNo. |
5142-23-4 |
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| Storage |
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| Shipping |
RoomtemperatureincontinentalUS;mayvaryelsewhere |
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| Solvent&Solubility |
DMSO:≥1.5mg/mL;DMSO:6.8mg/mL(Needultrasonic);H2O:8.82mg/mL(Needultrasonic) *"<1 mg/ml"="" means="" slightly="" soluble="" or="" insoluble.="" "≥"="" means="" soluble,="" but="" saturation=""> Purity:99.84% |
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