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产品详细
Norgen Biotek是来自加拿大的著名生物技术公司，生产基于技术上的核酸和蛋白质纯化及富集产品。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖。目前该公司的产品多达40余种，覆盖基因组和蛋白组，广泛地应用于不同的领域。
提纯RNA的方法一般有两种，离心法和有机抽取法（例如Trizol）。Norgen Biotek是世界上*家真正意义上的生产以离心柱（Spin Columns）为平台，并能纯化所有种类RNA（包括小分子RNA）的公司。
Norgen Biotek和其他公司生产的以离心纯化为原理的试剂盒比：
- 其他公司一般都使用硅材料（silica matrix）为填充物。硅材料的缺点是在没有酚存在的情况下，不能回收所有的RNA，使得其纯化效率很低，特别是对小RNA（例如microRNA）,其回收率几乎为零。这也是为什么很多客户在使用了其他公司的以离心为原理的总RNA纯化产品后，往往得不到预想结果的原因。
- 即使对少量细胞的总RNA提纯，也具有很高的线性（linearity on low numbers of cells）。在细胞数量多的情况下，其他公司的产品表现还可以，但是在细胞数量少的情况下，其纯化效率会大大降低，为提高效率往往需要通过其他的途径，例如Carrier RNA。Norgen的试剂盒可以从一个细胞中提纯出高质量的总RNA，在同行业中处于地位。
- Norgen的试剂盒完全满足所有的下游实验应用。
Norgen Biotek和其他公司生产的以Trizol原理的试剂盒比较：
- 在这些方法中，层析会造成有机试剂的残留，从而抑制许多下游实验的进行。
- 许多实验室由于健康和环境的因素，已经放弃了以酚抽为原理的提纯方法。
- 对大样品，使用Trizol的效果还可以，但同时也由于基因组DNA的增加，会阻止RNA的沉淀，从而间接地降低了纯化效率
- 对小量样品，使用Trizol的效果往往不佳。
- 使用Trizol中，乙醇沉淀（ethanol precipitation）效果的好坏和RNA的量有密切的关系，因此对于少量样品，沉淀法往往得不到zui终的RNA产物。
- 由于许多实验（e.g. qRT-PCR），并不需要很多量的RNA，因此Trizol方法已经逐渐被其他方法所代替。
Norgen Biotek采用的柱子填充物是一种新型的，并有保护的非硅胶材料。可以在没有酚等有害有机溶剂存在的情况下，纯化包括microRNA在内的所有RNA,而且其纯化回收效率高达99%。人们一直认为如果要纯化microRNA,必须要用Trizol，而不是离心法。这种论断已经过时。Norgen Biotek总RNA纯化试剂盒不仅避免Trizol带给实验者的健康损害，而且可以纯化出包括miroRNA在内的所有RNA。因此Norgen Biotek是一款真正意义上的绿色产品。
鉴于以上原因，Norgen被著名的microRNA表达谱分析公司Exiqon定为专有核酸提取供应商（请点击这里了解详情）。
更多关于Norgen 总RNA产品的特点介绍，请点击这里。
Norgen Biotek的RNA纯化产品包括：
| RNA Kits |
产品 |
货号 |
规格 |
| Total RNA Purification Kit | 17200 | 50 preps | |
| Animal Tissue Total RNA Purification Kit | 25700 | 50 preps | |
| Cytoplasmic & Nuclear RNA Purification Kit | 21000 | 50 preps | |
| Soil DNA Isolation Kit | 26500 | 50 preps | |
| Water RNA/DNA Purification Kit 0.22um | 26450 | 25 preps | |
| Water RNA/DNA Purification Kit 0.45um | 26450 | 25 preps | |
| Leukocyte RNA Purification Kit | 21200 | 50 preps | |
| microRNA Purification Kit | 21300 | 25 preps | |
| RNA/DNA/Protein Purification Kit | 23500 | 20 preps | |
| RNA/Protein Purification Kit | 23000 | 20 preps | |
| RNA CleanUp and Concentration Kit | 23600 | 50 preps | |
| Urine Exfoliated Cell RNA Purification Kit | 22500 | 20 preps | |
| Urine Bacteria RNA Purification Kit | 23400 | 20 preps | |
| All-in-One Purification Kit | 24200 | 20 preps | |
| FFPE RNA Purification Kit | 25300 | 50 preps | |
| FFPE RNA/DNA Purification Kit | 25000 | 50 preps | |
| Plant/Fungi RNA Purification Kit | 25800 | 50 preps | |
| Plant RNA/DNA Purification Kit | 24400 | 50 preps | |
| Total RNA Purification Kit （96-Well Format） | 24300 | 2 plates/192 preps | |
| RBC Lysis Solution | 21201 | 90 mL |
产品说明书
Total RNA纯化试剂盒
Total RNA纯化试剂盒能够快速地从培养的细胞，组织样品，血液，细菌，酵母，真菌，以及植物中提取和纯化total RNA。
- 纯化多种RNA，包括大片段的mRNA和rRNA,以及microRNA和siRNA等小片段
- 操作快速简单，能够在20分钟内可以完成多达10个样品的分离和纯化，为你节省宝贵的实验时间
- 无需酚和氯仿的抽提过程，为你保持一个绿色健康的实验环境
- 可以从很少量（比如，一个细胞中）的样品中提取total RNA,是你再不用为少量的样品而发愁
- 所纯化的total RNA可以广泛的应用于下游操作中，例如，定量PCR（real-time PCR），northen-blotting,RNAase protection和引物延伸，以及涉及RNA的基因芯片研究
简单快速的操作流程，在很短的时间里提纯出Total RNA,为您节省了宝贵的时间和精力
Norgen的total RNA纯化试剂盒可以从E.Coli中提纯出高质量的RNA。和其他公司的类似产品相比，Norgen试剂盒可以把小片段的RNA成功的分离出来
（红色圆圈所包括的条带）。
高敏感性。从递减的 293细胞中分离出的total RNA,经过RT-PCR所产生的条带
Total RNA纯化试剂盒（96孔板）:从96孔板中直接提取Total RNA
动物组织Total RNA纯化试剂盒:该产品能从所有动物组织中，包括富含纤维的肌肉和心脏中，提取总RNA。试剂盒里提供的Proteinase K可以有效地去除组织中的胶原，收缩蛋白，结缔组织。提纯出的RNA包括所有分子量大小的RNA，例如大mRNA,rRNA,以及microRNA,siRNA,并且不含有酚和氯仿等有害物质，可以直接用于下游实验，例如，Real time PCR,逆转录PCR,Northern blotting,RNAase protection,引物延伸和芯片检测。
microRNA纯化试剂盒: 能够从培养的细胞，小的组织样品，以及细菌中快速纯化小于200nt的全部小RNA分子，例如miRNA，siRNA，tRNA,和5S rRNA等。
- 操作快速简易。能够在30分钟内完成10个样品的提取和纯化
- 无需酚和氯仿的抽提过程，为你保持一个健康的实验环境
- 无大片段RNA和基因组DNA的污染• 所纯化的RNA可以广泛的应用于下游操作中，例如，Northern blotting,microarray analysis等。
简单快速的操作流程，使您在很短的时间里提纯出micro RNA
| 分类 | 货号 | 品名 | 规格 | 目录价 | 起订量 |
| RNA Kits | 17200 | Total RNA Purification Kit | 50 preps | ￥2,940.00 | |
| 37500 | Total RNA Purification Kit | 100 preps | ￥5,523.00 | ||
| 24300 | Total RNA Purification Kit - 96 well format | 2 plates | ￥8,526.00 | ||
| 26800 | Total RNA Purification Maxi Kit - 8 preps | 8 preps | ￥1,764.00 | ||
| 48300 | Total RNA Purification Plus Kit （50） | 50 preps | ￥3,486.00 | ||
| 48400 | Total RNA Purification Plus Kit （100） | 100 preps | ￥6,132.00 | ||
| 25700 | Animal Tissue RNA Purification Kit | 50 preps | ￥4,042.60 | ||
| 35300 | Total RNA Purification Micro Kit - 50 preps | 50 preps | ￥3,465.00 | ||
| 36200 | Fatty Tissue RNA Purification Kit | 25 preps | ￥2,415.00 | ||
| 21000 | Cytoplasmic & Nuclear RNA Purification Kit | 50 preps | ￥3,675.00 | ||
|
37400 |
Cytoplasmic & Nuclear RNA Purification Kit | 100 preps | ￥6,993.00 | ||
| 21200 | Leukocyte RNA Purification Kit | 50 preps | ￥3,675.00 | ||
| 37800 | Leukocyte RNA Purification Kit - 96 Well | 2 plates | ￥9,187.60 | ||
| 23400 | Urine Bacteria RNA Purification Kit | 20 preps | ￥2,058.00 | ||
| 47200 | Urine Exosome RNA Purification Kit | 50 preps | ￥5,260.00 | ||
| 49200 | Plasma/Serum Exosome RNA Purification Kit | 50 preps | ￥5,260.00 | ||
| 22500 | Urine （exfoliated cell） RNA Purification Kit | 20 preps | ￥2,352.00 | ||
| 29000 | Urine microRNA Purification Kit | 25 preps | ￥2,940.00 | ||
| 21300 | microRNA Purification Kit | 25 preps | ￥2,415.00 | ||
| 24400 | Plant RNA/DNA Purification Kit | 50 preps | ￥2,940.00 | ||
| 25300 | FFPE RNA Purification Kit | 50 preps | ￥4,042.60 | ||
| 25400 | FFPE RNA Purification Kit （96-well） | 2 plates | ￥9,996.00 | ||
| 25000 | FFPE RNA/DNA Purification Kit | 50 preps | ￥3,675.00 | ||
| 23600 | RNA Cleanup and Concentration Kit | 50 preps | ￥2,940.00 | ||
| 43200 | RNA Cleanup and Concentration Kit | 100 preps | ￥5,523.00 | ||
| 25100 | RNA Cleanup and Concentration Kit 96 well | 2 plates | ￥8,526.00 | ||
| 21201 | RBC Lysis Solution | 90 mL | ￥735.00 | ||
| 21205 | RBC Lysis Solution | 500 mL | ￥3,675.00 | ||
| 21207 | RBC Lysis Solution | 1000 mL | ￥6,720.00 | ||
| 25800 | Plant/Fungi RNA Purification Kit | 50 preps | ￥3,675.00 | ||
| 31300 | Plant/Fungi RNA Purification Kit 96 preps | 96 preps | ￥7,056.00 | ||
| 31900 | Plant/Fungi RNA Purification Kit 96 well format | 2 plates | ￥9,261.00 | ||
| 42300 | Plant RNA Purification Kit - Maxi format | 8 preps | ￥2,719.60 | ||
| 24200 | All-in-One Purification Kit | 20 preps | ￥3,675.00 | ||
| 23500 | RNA/DNA/Protein Purification Kit - 20 Preps | 20 preps | ￥2,940.00 | ||
| 24000 | RNA/DNA/Protein Purification Kit - 50 Preps | 50 preps | ￥6,247.60 | ||
| 47800 | RNA/DNA/Protein Purification Plus Kit - 20 Preps | 20 preps | ￥3,206.00 | ||
| 47700 | RNA/DNA/Protein Purification Plus Kit - 50 Preps | 50 preps | ￥6,930.00 | ||
| 48600 | RNA/DNA Purification Kit - 20 preps | 20 preps | ￥2,716.00 | ||
| 48700 | RNA/DNA Purification Kit - 50 preps | 50 preps | ￥5,866.00 | ||
| 50300 | RNA/DNA Purification Micro Kit | 50 preps | ￥6,240.00 | ||
| 23000 | RNA/Protein Purification Kit - 20 preps | 20 preps | ￥2,415.00 | ||
| 24100 | RNA/Protein Purification Kit - 50 preps | 50 preps | ￥5,292.00 | ||
| 48100 | RNA/Protein Purification Plus Kit - 20 preps | 20 preps | ￥2,716.00 | ||
| 48200 | RNA/Protein Purification Plus Kit - 50 preps | 50 preps | ￥5,866.00 | ||
| 23800 | CleanAll Purification Kit | 20 preps | ￥2,940.00 | ||
| 26450 | Water RNA/DNA Purification Kit - 0.45 um | 25 preps | ￥5,544.00 | ||
| 26400 | Water RNA/DNA Purification Kit - 0.22 um | 25 preps | ￥5,817.00 | ||
| 29600 | Urine Total RNA Purification Maxi Kit - slurry format | variable | ￥5,817.00 | ||
| 32800 | Viroid RNA Purification Kit | 50 preps | ￥4,042.60 | ||
| 41900 | Viroid RNA Purification 96-Well Kit | 2 plates | ########## | ||
| 27700 | Soil RNA Purification Kit | 25 preps | ￥3,234.00 | ||
| 49400 | Stool RNA Purification Kit - 25 preps | 25 preps | ￥2,866.60 | ||
| 49500 | Stool RNA Purification Kit - 50 preps | 50 preps | ￥4,900.00 | ||
| 35800 | Yeast/Fungal RNA/DNA Purification Kit - 50 preps | 50 preps | ￥3,465.00 | ||
| 25710 | RNase-Free DNase I Kit | 50 reactions | ￥1,470.00 | ||
| 48900 | Blood RNA/DNA Preservation and Isolation Kit | 20 preps | ￥3,430.00 | ||
| 43400 | Preserved Blood RNA Purification Kit I （for use with TempusTM Blood RNA Tubes） | 50 preps | ￥5,250.00 | ||
| 43500 | Preserved Blood RNA Purification Kit II （for use with PaxgeneTM Blood RNA Tubes） | 50 preps | ￥5,250.00 | ||
| Protein Kits | 10100 | ProteoSpin CBED Micro Kit | 25 preps | ￥2,940.00 | |
| 10400 | ProteoSpin CBED Micro Kit | 50 preps | ￥4,630.60 | ||
| 17000 | ProteoSpin CBED Maxi Kit | 4 preps | ￥1,617.00 | ||
| 10200 | ProteoSpin Detergent Clean-Up Micro Kit | 25 preps | ￥2,940.00 | ||
| 10500 | ProteoSpin Detergent Clean-Up Micro Kit | 50 preps | ￥4,630.60 | ||
| 17100 | ProteoSpin Detergent Clean-Up Maxi Kit | 4 preps | ￥1,617.00 | ||
| 10300 | ProteoSpin Inclusion Body Isolation Micro Kit | 20 preps | ￥3,255.00 | ||
| 10600 | ProteoSpin Inclusion Body Isolation Micro Kit | 50 preps | ￥5,218.60 | ||
| 17700 | ProteoSpin Inclusion Body Isolation Maxi Kit | 4 preps | ￥1,837.60 | ||
| 17300 | ProteoSpin Abundant Serum Protein Depletion Kit | 25 preps | ￥2,940.00 | ||
| 17400 | ProteoSpin Urine Protein Concentration Kit | 25 preps | ￥2,940.00 | ||
| 21600 | ProteoSpin Urine Protein Concentration MAXI Kit | 4 preps | ￥1,732.60 | ||
| 17500 | ProteoSpin On-Column Proteolytic Digestion Kit | 25 preps | ￥2,940.00 | ||
| 18700 | Inclusion Body Solubilization Reagent | 25 mL | ￥582.80 | ||
| 18701 | Inclusion Body Solubilization Reagent | 100 mL | ￥1,890.00 | ||
| 18800 | Cell Lysis Reagent | 100 mL | ￥1,023.80 | ||
| 18801 | Cell Lysis Reagent | 500 mL | ￥1,890.00 | ||
| 30300 | Detergent Free Total Protein Purification Kit | 25 preps | ￥2,940.00 | ||
| 22000 | ProteoSpin Total Protein Concentration Kit | 25 preps | ￥2,940.00 | ||
| 23300 | ProteoSpin Total Protein Detergent CleanUp Kit | 20 preps | ￥2,940.00 | ||
| 22800 | ProteoSpin Endotoxin Removal Micro Kit - For Proteins and Peptides | 20 preps | ￥2,667.00 | ||
| 22200 | ProteoSpin Endotoxin Removal Maxi Kit - For Proteins and Peptides | 4 preps | ￥1,638.00 | ||
| 43600 | ProteoSpin Endotoxin Removal Maxi Kit - For Proteins and Peptides | 24 preps | ￥9,072.00 | ||
| DNA Kits | 13300 | Plasmid MiniPrep Kit | 50 preps | ￥1,102.60 | |
| 46500 | Plasmid MaxiPrep Kit （4 Preps） | 4 preps | ￥1,407.00 | ||
| 46600 | Plasmid MaxiPrep Kit （20 Preps） | 20 preps | ￥6,320.00 | ||
| 25900 | Plasmid MiniPrep Kit - 96 well x 1 | 1 plate | ￥3,241.40 | ||
| 43300 | Plasmid MiniPrep Kit - 96 well x 4 | 4 plates | ########## | ||
| 14400 | PCR Purification Kit | 50 preps | ￥1,176.00 | ||
| 45700 | PCR Purification Kit （250） | 250 preps | ￥5,586.00 | ||
| 24800 | PCR Purification Kit - 96 well format | 2 plates | ￥5,145.00 | ||
| 34400 | Sequencing Reaction Clean-Up Kit （96 well） | 2 plates | ￥5,145.00 | ||
| 34500 | Sequencing Reaction Clean-Up Kit | 50 preps | ￥2,058.00 | ||
| 13100 | DNA Gel Extraction Kit | 50 preps | ￥1,176.00 | ||
| 17900 | Bacterial Genomic DNA Isolation Kit | 50 preps | ￥1,470.00 | ||
| 18600 | Yeast Genomic DNA Isolation Kit | 50 preps | ￥1,470.00 | ||
| 26300 | Yeast Genomic DNA Isolation Kit - 96 Well Format | 2 plates | ￥7,276.60 | ||
| 27300 | Fungi/Yeast Genomic DNA Isolation Kit | 50 preps | ￥2,058.00 | ||
| 24700 | Genomic DNA Isolation Kit （50） | 50 preps | ￥1,470.00 | ||
| 17800 | Low Copy Plasmid MiniPrep Kit | 25 preps | ￥1,102.60 | ||
| 18050 | BAC DNA MiniPrep Kit | 50 preps （1.5 mL culture） | ￥1,680.00 | ||
| 18100 | Urine DNA Isolation Kit | 50 preps | ￥3,675.00 | ||
| 48800 | Urine DNA Isolation Kit （slurry format） | 50 preps | ￥6,300.00 | ||
| 50100 | Urine DNA Isolation Maxi Kit （slurry format） | 50 preps | ￥7,700.00 | ||
| 27100 | Urine DNA Isolation 96-Well Kit （Slurry Format） | 1 plate | ￥6,615.00 | ||
| 47050 | Urine DNA Isolation Kit for Exfoliated Cells or Bacteria | 50 preps | ￥2,060.00 | ||
| 22700 | Endotoxin Removal Kit （Mini） - For DNA | 20 preps | ￥2,667.00 | ||
| 21900 | Endotoxin Removal Kit （Maxi） - For DNA | 4 preps | ￥1,638.00 | ||
| 45400 | Saliva DNA Isolation Kit （50 Prep） | 50 preps | ￥1,750.00 | ||
| 35200 | Saliva DNA Isolation 96-Well Kit - 2 96-Well Plates, 192 Preps | 2 x 96 wells | ￥6,580.00 | ||
| 21500 | Milk Bacterial DNA Isolation Kit | 25 preps | ￥1,911.00 | ||
| 46700 | Phage DNA Isolation Kit | 25 preps | ￥2,415.00 | ||
| 46800 | Phage DNA Isolation Kit | 50 preps | ￥4,550.00 | ||
| 25600 | Direct Fungi DNA Isolation Kit | 25 preps | ￥2,058.00 | ||
| 47400 | FFPE DNA Isolation Kit | 50 preps | ￥2,600.00 | ||
| 26200 | Plant/Fungi DNA Isolation Kit | 50 preps | ￥2,352.00 | ||
| 26900 | Plant/Fungi DNA Isolation Kit （96-well） | 2 plates | ￥7,276.60 | ||
| 26500 | Soil DNA Isolation Kit | 50 preps | ￥2,940.00 | ||
| 49300 | Soil DNA Isolation Kit （High Humic Acid） | 50 preps | ￥3,080.00 | ||
| 43700 | Soil DNA Isolation 96-Well Kit （Slurry Format） | 1 x 96 wells | ￥9,660.00 | ||
| 27600 | Stool DNA Isolation Kit | 50 preps | ￥2,940.00 | ||
| 34100 | Oligo Clean-Up and Concentration Kit | 50 preps | ￥2,940.00 | ||
| 36000 | Dried Blood Spot （DBS） Genomic DNA Isolation Kit | 50 preps | ￥2,583.00 | ||
| 35900 | Dried Blood Spot （DBS） Genomic DNA Isolation Kit （For Resource Limited Settings） | 50 preps | ￥2,940.00 | ||
| 44800 | Milk DNA Preservation and Isolation Kit | 25 preps | ￥2,940.00 | ||
| DNA Ladders | 11200 | Mini Sizer 50bp DNA Ladder | 100 loads | ￥735.00 | |
| 13525 | 50 bp DNA Ladder | 100 loads | ￥735.00 | ||
| 11300 | PCR Ranger 100bp DNA Ladder | 100 loads | ￥735.00 | ||
| 11400 | PCR Sizer 100bp DNA Ladder | 100 loads | ￥735.00 | ||
| 12800 | FastRunner DNA Ladder | 100 loads | ￥735.00 | ||
| 11500 | LowRanger 100bp DNA Ladder | 100 loads | ￥735.00 | ||
| 11600 | Clone Sizer 100bp DNA Ladder | 100 loads | ￥735.00 | ||
| 11700 | MidRanger 1kb DNA Ladder | 100 loads | ￥735.00 | ||
| 11800 | FullRanger 100bp DNA Ladder | 100 loads | ￥735.00 | ||
| 11900 | HighRanger 1kb DNA Ladder | 100 loads | ￥735.00 | ||
| 12000 | HighRanger Plus 100bp DNA Ladder | 100 loads | ￥735.00 | ||
| 12100 | UltraRanger 1kb DNA Ladder | 100 loads | ￥735.00 | ||
| 11215 | Mini Sizer 50bp DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 13527 | 50 bp DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 11315 | PCR Ranger 100bp DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 11415 | PCR Sizer 100bp DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 12815 | FastRunner DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 11515 | LowRanger 100bp DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 11615 | Clone Sizer 100bp DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 11715 | MidRanger 1kb DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 11815 | FullRanger 100bp DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 11915 | HighRanger 1kb DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 12015 | HighRanger Plus 100bp DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| 12115 | UltraRanger 1kb DNA Ladder | 5 x 100 loads | ￥3,360.00 | 10 | |
| RNA Ladders | 15002 | 100b RNA Ladder | 50 loads | ￥1,134.00 | |
| 15003 | 1kb RNA Ladder | 50 loads | ￥1,134.00 | ||
| 15006 | 20 |
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