Rabbit polyclonal antibody

Antigen/Purification: ExpandCollapse

The antigen is a peptide from the subunit region of the voltage gated potassium channel, Kv2.2 of rat and Xenopus, conjugated to keyhole limpet hemocyanin (KLH).

The antibody is prepared from pooled rabbit serum by affinity purification using a Sulfo-Link® column matrix to which the peptide immunogen was coupled.

Biological Significance: ExpandCollapse

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).

Storage

100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.

Product Specific References

Gravagna, N.G., Knoeckel, C.S., Taylor, A.D., Hultgren, B.A. and Ribera, A.B., 2008. Localization of Kv2. 2 protein in Xenopus laevis embryos and tadpoles. Journal of Comparative Neurology, 510(5), pp.508-524. PMID: 18680201

D. Guan, T. Tkatch, D. J. Surmeier, W. E. Armstrong, and R. C. Foehring (2007) Kv2 subunits underlie slowly inactivating potassium current in rat neocortical pyramidal neuronsJ. Physiol., Jun 2007; 581: 941 – 960. PMID: 17379638