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Bpsbioscience/Mouse Sphingosine kinase 2, His-tag/40614/10 µg
2025-11-09
Species: Mouse Host Species: Sf9 insect cells MW: 68 kDa Genbank #: NM_203280 Tag(s): N-terminal His-tag a.a: full length Description:Mouse Sphingosine kinase 2 (GenBank Accession No. NM_203280), full length with N-terminal His-tag, MW=68 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Synonym(s): SphK2, sphingosine kinase 2, SPK2 Specific Activity: 2 U/µg Unit Definition: A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P per minute at 37°C. Assay Conditions: Sphingosine kinase activity was determined using D-erythro-sphingosine and ATP as substrates. Assay condition: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 4 µM D-erythrosphingosine, and 5 ug/ml mouse sphingosine kinase 2 at 37°C for 30 min. ATP reduction is detected using Kinase-Glo® Luminescent Kinase Assay Platform (Promega Corporation, Madison, WI). Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. Format: Aqueous buffer solution Storage / Stability: >6 months at –80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Zemann, B. et al., Blood.107(4):1454-8 (2006) 2. Maceyka, M. et al., J Biol Chem. 280(44):37118-29 (2005) Warning(s): Avoid freeze/thaw cycles Scientific Category: Kinase/Lipids Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.
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