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FlexAble 抗体标记试剂盒

跳转至：一般说明标准工作流程多重处理常见问题  FlexAble 抗体标记试剂盒的优势：简单的 2 步实验方案标记可在 10 分钟内完成仅需 30 秒的动手操作时间无需准备，也无需特殊设备抗体可在缀合后立即使用

 

FlexAble 抗体标记试剂盒盒中有什么？FlexLinkerFlexQuencherFlexBuffer

 

发货后，-20°C 下可稳定保存 1 年，+4°C 下可稳定保存 6 个月。避免暴露在光线下。

 

下载 PDF 格式的实验方案

流式细胞术简介

流式细胞术是一种用于分析样本群体中每个细胞特性的技术。

跳转至：流式细胞术简介流式细胞术应用流式细胞术荧光团和染料流式细胞术多色面板设计流式细胞术样品制备研究细胞外和细胞内蛋白质的实验方案研究细胞活力和细胞凋亡的实验方案流式细胞术的热门抗体概述

流式细胞术是一种能够测量异源群体中单个颗粒（细胞）特性的技术。这使得研究人员能够根据这些特性对每个细胞进行表型分析，在某些情况下还可以将细胞分选为不同的亚群，以便在称为荧光激活细胞分选 (FACS) 的专门流式细胞术中进行进一步评估。在流式细胞仪中，悬浮细胞被迫排成\"单列”，然后通过激光，从而能够测量f 颗粒大小和粒度。通过使用与细胞表面或细胞内的靶蛋白结合的荧光偶联抗体进行进一步的表型分析，从而能够测量荧光强度作为另一个参数。

流式细胞术如何工作？

1.首先，细胞悬浮液流过腔室，然后通过流体系统将其分离成单细胞流。

2.然后溶液通过激光束或一组激光器（图 1 - 流式细胞术概述）。

3.一组透镜、滤光片和二向色镜用于收集和分离光线。

4.光线以 90 度角收集（侧向散射 - SSC）并与细胞流直接相反（前向散射 - FSC）。

5.使用光电二极管或光电倍增管 (PMT) 检测光。

除了这些固有的物理特性之外，还可以以类似的方式测定标记物的荧光。一些流式细胞仪可以lso 根据这些属性的用户门控条件对单元格进行排序。这种类型的流式细胞术通常称为荧光激活细胞分选 (FACS)。

图 1 - 流式细胞术概述

流式细胞术应用       质膜受体表达分析细胞活力、凋亡和坏死分析       细胞表型分析       细胞周期分析       细胞分选       细胞内蛋白表达分析       生物标志物检测       细胞计数Validation Data GalleryWB analysis using 55004-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.View All Images (6)×Various lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.FC experiment of Jurkat using 55004-1-AP1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human Cyclin B1 (55004-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .View All Images (6)×1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human Cyclin B1 (55004-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .IP experiment of HeLa using 55004-1-APIP Result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:500) with HeLa cells lysate 1200ug.View All Images (6)×IP Result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:500) with HeLa cells lysate 1200ug. -/ - Cat No. 55004-1-AP Freight/Packing: - Country/Region Size/Concentration Quantity Add to Basket Add to Quote Please visit your regions distributor: Proteintech Guarantee Request bulk or custom quote Chat with a team member The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note. Learn More Positive WB detected inHeLa cells, NIH/3T3 cells, PC-3 cells, HepG2 cells, Jurkat cells, K-562 cellsPositive IP detected inHeLa cellsPositive FC detected inJurkat cells Recommended dilutionApplicationDilutionWestern Blot (WB)WB : 1:1000-1:4000Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WBSample-dependent, check data in validation data gallery 55004-1-AP targets Cyclin B1 in WB, IP, IF, FC,ELISA applications and shows reactivity with human, mouse samples. Calculated molecular weight 48 kDaObserved molecular weight 55-60 kDaGenBank accession numberNM_031966 Gene symbol CCNB1 Background InformationCyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation. ProtocolsProduct Specific ProtocolsWB protocol for Cyclin B1 antibody 55004-1-APDownload protocolIP protocol for Cyclin B1 antibody 55004-1-APDownload protocolFC protocol for Cyclin B1 antibody 55004-1-APDownload protocolStandard ProtocolsClick here to view our Standard Protocols PublicationsAll (151)WB (142)IHC (3)IF (1)SpeciesApplicationTitlemouseWBAging (Albany NY)The ubiquitin ligase KBTBD8 regulates PKM1 levels via Erk1/2 and Aurora A to ensure oocyte quality.Authors - Yan-Ru LiView ArticlehumanWBCancers (Basel)PMID: 30754729Authors - Hui-Wen TsengView ArticlehumanWBBiomed PharmacotherLin28 promotes dental pulp cell proliferation via upregulation of cyclin-dependent proteins and interaction with let-7a/IGF2BP2 pathways.Authors - Yan LiuView ArticlehumanWBSci RepLow C6orf141 Expression is Significantly Associated with a Poor Prognosis in Patients with Oral Cancer.Authors - Cheng-Mei YangView ArticlehumanWB,IHCOnco Targets TherPrognostic implications and oncogenic roles of MYBL2 protein expression in esophageal squamous-cell carcinoma.Authors - Hui QinView ArticlehumanWBJ Exp Clin Cancer ResNuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway.Authors - Zizhuo LiView Article See more citations Related ProductsCDK1-Specific Rabbit Polyclonal antibody19532-1-APCyclin B2 Rabbit Polyclonal antibody21644-1-APCyclin A2 Rabbit Polyclonal antibody18202-1-APPLK1 Rabbit Polyclonal antibody10305-1-APCDC25C Rabbit Polyclonal antibody16485-1-APCyclin D1 Mouse Monoclonal antibody60186-1-IgP21 Rabbit Polyclonal antibody10355-1-APP53 Rabbit Polyclonal antibody10442-1-APCDK2 Rabbit Polyclonal antibody10122-1-APCyclin G Rabbit Polyclonal antibody10897-1-AP WB FiguresWB analysis of HeLa using 55004-1-APHeLa cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.WB analysis using 55004-1-APVarious lysates were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB analysis of NIH/3T3 using 55004-1-AP NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (Cyclin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB analysis of PC-3 using 55004-1-APPC-3 cells were subjected to SDS PAGE followed by western blot with 55004-1-AP (CCNB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.IP FiguresIP experiment of HeLa using 55004-1-APIP Result of anti-Cyclin B1 (IP:55004-1-AP, 4ug; Detection:55004-1-AP 1:500) with HeLa cells lysate 1200ug.FC FiguresFC experiment of Jurkat using 55004-1-AP1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human Cyclin B1 (55004-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH . \"Cyclin B1 antibodies\" comparison At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs. We have selected the top cited antibodies from these vendors for you to compare.