Overview:

Label exosomes with luciferase using XPack^TM

Easily create stable cell lines that produce luciferase-labeled exosomes with the XPack MSCV-XP-Luciferase-EF1α-Puro Expression Lentivector. Take advantage of SBI’s well-proven lentivector technology to express luciferase from the strong MSCV promoter (also available with a CMV promoter for expression in hematopoietic and stem cells) and the optimized XPack exosome targeting tag to package luciferase into exosomes.

The XPack System at a glance:

• Powerful—enables a wealth of applications, including
  • Exosome labeling
  • Exosome cargo tracking
  • Exosomes as cargo delivery vehicles
  • Exosomes as biotherapeutics
  • Exosome-generating stable cell lines with protein cargo-of-interest
• Optimized—SBI developed the XPack peptide tag for robust exosome targeting
• Reliable—based on SBI’s highly-cited, well-proven lentivector technology
• Versatile
  • Two promoter options for expression in a wide range of cells
  • Comes as pre-built reporters (GFP, RFP, luciferase) or ready-to-customize cloning lentivectors

Choose the XPack Lentivector that’s right for you

For most cell lines

For hematopoietic and stem cell lines

Supporting Data:

XPack efficiently packages luciferase into exosomes which can be used to deliver GFP to target cells

Part 1: Packaging luciferase into exosomes with XPack

The XPack-Luciferase fusion protein is efficiently packaged as exosome cargo and delivered to target cells. HEK-293 cells were transfected with a plasmid encoding an N-terminal XPack-Luciferase fusion protein (Cat.# XPAK532PA-1). Forty-eight hours later, exosomes were isolated using ExoQuick-TC^® and luciferase levels quantified and compared to exosomes isolated from untransfected cells (Figure 1). Only XPack-Luciferase exosomes show robust luminescence indicating efficient packaging of luciferase into exosomes.

Figure 1. XPack efficiently packages luciferase into exosomes.

Part 2: XPack-Luciferase Exosome Delivery to Target Cells

The XPack-Luciferase exosomes isolated as described above were then added to recipient HEK-293 cells to test for luciferase delivery. After 24-hours, robust luminescence from cells exposed to XPack-Luciferase exosomes shows delivery of the luciferase cargo to the target cells (Figure 2). In contrast, untreated cells or cells treated with exosomes from untransfected cells show no luminescence.

Figure 2. XPack-Luciferase exosomes deliver luciferase to target cells.