Product Description

Pooled Normal Human Serum

Innovative Researchs Pooled Normal Human Serum is derived from whole blood which was obtained from FDA-approved facilities. The blood sample is allowed to clot, centrifuged, and the serum is isolated.The material undergoes viral testing, and the final product is aliquoted to customer specifications. All lots have been tested by an FDA-approved method and found to be negative for Human Immunodeciency Virus RNA (HIV-1 RNA), Antibodies to Human Immunodeciency Virus (Anti-HIV 1/2), Antibodies to Hepatitis C Virus (HCV), Hepatitis C Virus RNA (HCV RNA), non- reactive for Hepatitis B Surface Antigen (HbsAg), and Non-reactive by a screening test for syphilis. Bulk pricing is available - please inquire for details. This Innovative Research product is for in vitro research use only, is not to be consumed by animals or humans, and should be handled with general precaution as if capable of spreading infectious disease. Not for use in products subject to license under section 351 of the public health service act.

References:

(6-21-17)

Alpha-Gal Inactivated Heart Valve Bioprostheses Exhibit an Anti-Calcification Propensity Similar to Knockout Tissues

F Naso, U Stefanelli, E Buratto, G Lazzari, A Perota… - Tissue Engineering Part A, 2017... Address correspondence to: Filippo Naso, BSc. Biocompatibility Innovation. Medical DeviceBiocompatibility Laboratory Padova 35042. Italy E-mail ... Samples were subsequently incubatedin pooled normal human serum (Innovative Research, Peary Court Novi, MI) with 2 ... (3-9-17)

Defining the protective functions of broadly-reactive antibodies against influenza A virus

W He - 2016... for science, enthusiasm for knowledge and dedication for innovation are contagious. ... Human serum samples were obtained from Innovative Research (Novi, Michigan) and were collected between ...

(11-15-16)

Characterisation of successive Acinetobacter baumannii isolates from a deceased haemophagocytic lymphohistiocytosis patient

HJ Choi, MC Kil, JY Choi, SJ Kim, KS Park, YJ Kim... - International Journal of , 2016 (Innovative Research, Inc., Novi, MI) was determined using a previously described method 13 with modifications. Serum susceptibilities were determined(9-13-16)

Periodontal regeneration in swine after cell injection and cell sheet transplantation of human dental pulp stem cells following good manufacturing practice

J Hu, Y Cao, Y Xie, H Wang, Z Fan, J Wang, C Zhang - Stem Cell Research & , 2016... Corporation, Lakewood, NJ, USA), CELLstart, EZPassage Tool, HBSS-Ca/Mg free, D/F12, TrypLE, xeno-free B27, N2 supplement, MSCGM-CD and ProFreeze CDM (Invitrogen/Gibco, Carlsbad, CA, USA), human serum (Innovative Research, Inc., Novi, Michigan, USA), basic...

(6-14-16)

Painting factor H onto mesenchymal stem cells protects the cells from complement-and neutrophil-mediated damage

Y Li, W Qiu, L Zhang, J Fung, F Lin - Biomaterials, 2016 ... media under standard culturing conditions. Pooled normal human serum (NHS) were ordered from Innovative Research Inc (Novi, MI), and aliquoted NHS were stored in a -80C freezer until needed. (11-23-15)

Globally Optimized Targeted Mass Spectrometry (GOT-MS): Reliable Metabolomics Analysis with Broad Coverage

H Gu, P Zhang, J Zhu, D Raftery - Analytical Chemistry, 2015... To develop GOT-MS, pooled human serum samples were purchased from 106 Innovative Research, Inc. (Novi, MI). For the CRC metabolomics study, serum samples 107 from 18 CRC patients and 20 healthy controls were selected from those involved in our 108 ...

(10-3-15)

Serum levels of phytoestrogens as biomarkers of intake in Mexican women

MR Robles-Burgeo, MI Ortega-Vlez - International Journal of..., 2015... HPLC grade. Sodium acetate and formic acid were of reagent grade with a purity of 99% and 95%, respectively. Pooled human blank serum was purchased from Innovative Research (Novi, MI). Study subjects and study design. ... (8-24-15)

Interactions between ibrutinib and anti-CD20 antibodies; competing effects on the outcome of combination therapy

M Skarzynski, CU Niemann, YS Lee, S Martyr, I Maric - Clinical Cancer Research, 2015...for two hours in the presence of 33% normal human serum (Innovative Research, Novi, MI) as a source of complement. The proportion of lysed CD19...

(8-23-15)

Loss of hypermucoviscosity and increased fitness cost in colistin-resistant Klebsiella pneumoniae ST23 strains

MJ Choi, KS Ko - Antimicrobial Agents and Chemotherapy, 2015... (3) Bacterial suspensions containing 1.5 106 CFU/mL were collected from mid-log phase cultures, mixed at a 1:3 (vol/vol) ratios with pooled human serum (Innovative Research Inc.; Novi, MI, USA), and incubated at 37C. Colony counts were obtained for the ... (8-18-15)

The Effect of PON1 Enhancers on Reducing Acetylcholinesterase Inhibition Following Organophosphate Anticholinesterase Exposure in Rats

EC Meek, HW Chambers, RB Pringle, JE Chambers - Toxicology, 2015... Commercially available pooled human serum was obtained from Innovative Research, Novi, MI. 2.1. Organophosphate substrate synthesis. ...

(7-20-15)

Enhanced immunogenicity of a respiratory syncytial virus (RSV) F subunit vaccine formulated with the adjuvant GLA-SE in cynomolgus macaques

K Patton, S Aslam, C Shambaugh, R Lin, D Heeke - Vaccine, 2015... Test samples and positive control sera (IPLA-SER, Innovative Research, Novi MI) were heat inactivated, initially diluted 1:10, and 3-fold serially diluted in virus growth medium (VGM), comprised of minimum essential medium containing Earles balance salt solution, 1 non ...(5-23-15)

Exosome Enrichment of Human Serum using Multiple Cycles of Centrifugation

J Kim, Z Tan, DM Lubman - ELECTROPHORESIS, 2015... David M. Lubman, Ph.D. Maude T. Lane Professor of Surgery University of Michigan Medical Center Department of Surgery MSRB I, A510B ... 2. Materials and Methods 2.1. Materials Pooled normal human serum samples were obtained from Innovative Research (Novi, MI, USA).

(4-29-15)

Noninvasive Visualization of Tumoral Fibrin Deposition Using a Peptidic Fibrin-binding Single Photon Emission Computed Tomography Tracer

LWE Starmans, T van Mourik, R Rossin, I Verel - Molecular Pharmaceutics, 2015...per group) was incubated with 800 L of mouse serum (non-Swiss albino from C57BL/6; Innovative Research Inc., Novi, MI, USA) or human serum (Innovative Research Inc.) for up to 24 h. At 1, 3, 8 and 24 h time points, 200...(4-15-15)

Chromatographic separation of PTAD-derivatized 25-hydroxyvitamin D 3 and its C-3 epimer from human serum and murine skin

MD Teegarden, KM Riedl, SJ Schwartz - Journal of Chromatography B, 2015...Human serum and pooled umbilical cord serum were acquired from Innovative Research (Novi,MI)...in this work satisfies the needs of emerging areas of vitamin D research, as it...

(3-8-15)

Massive Glutamine Cyclization to Pyroglutamic Acid in Human Serum Discovered Using NMR Spectroscopy

GAN Gowda, YN Gowda, D Raftery - Analytical Chemistry, 2015Pooled human serum (100 mL) was procured from Innovative Research, Inc. Page 4 of 21 ...Page 5. 5 (Novi, MI) and shipped to Seattle under dry ice, where it was subsequently thawed and aliquoted into 2 mL Eppendorf tubes and frozen at -80...(1-21-15)

Hydrastine Pharmacokinetics and Metabolism after a Single Oral Dose of Goldenseal (Hydrastis canadensis) to Humans

PK Gupta, GW Barone, B Gurley, EK Fifer - Drug Metabolism and , 2015Each goldenseal was independently determined to contain 13 mg of hydrastine. Human serum was purchased from Millipore (Bedford, MA) or Innovative Research (Novi, MI). LCMS Optima

(1-14-15)

Broadly-Neutralizing Anti-Influenza Virus Antibodies: Enhancement of Neutralizing Potency in Polyclonal Mixtures and IgA Backbones

W He, CE Mullarkey, JA Duty, TM Moran, P Palese - Journal of Virology, 2015MATERIALS AND METHODS 101 Human serum samples 102 Human serum samples were obtained from Innovative Research (Novi, Michigan) and were collected 103 between March 2012 and April 2013. Samples were pre-screened using HAI assay against wild type 104(1-12-15)

A Novel Antibody Engineering Strategy for Making Monovalent Bispecific Heterodimeric IgG Antibodies by Electrostatic Steering Mechanism

Z Liu, EC Leng, K Gunasekaran, M Pentony, M Shen - Journal of Biological , 2015From the 1. Department of Therapeutic Discovery, 2. Department of Process & Product Development, and 3. Therapeutic Innovation Unit of 4Amgen Inc. Pooled normal human serum(cat IPLA-SER) was purchased from Innovative Research, Inc. (Novi, MI).

(12-25-14)

Improved profiling of estrogen metabolites by orbitrap LC/MS

X Li, AA Franke - Steroids, 2014Serum sample preparation (unconjugated forms). Commercial pooled normal human serum (Innovative Research, Novi, MI) was used as quality control samples. No estrogen metabolites were detected in this serum except E1 and 2OHE1 at low levels (data not shown).(11-26-14)

Expanding the Limits of Human Blood Metabolite Quantitation using NMR Spectroscopy

GAN Gowda, YN Gowda, D Raftery - Analytical Chemistry, 2014Pooled human serum was obtained from Innovative Research, Inc. (Novi, MI). Deionized (DI) water was purified using an in-house Synergy Ultrapure Water System from Millipore (Billerica, MA).

(11-18-14)

Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential

I Adini, A Adini, L Bazinet, RS Watnick, DR Bielenberg - The FASEB Journal, 2014Human serum was purchased from Innovative Research (Novi, MI, USA). Biochemical methods ELISA was performed with Quantikine Human or Mouse MCP- 1 ELISA kits (R&D Systems).(9-28-14)

Quantification of complement system activation by measuring C5b-9 cell surface deposition using a cell-ELISA technique

H Jeon, JS Lee, S Yoo, MS Lee - Journal of Immunological Methods, 2014 Each cell line was seeded at 10,000 cells/well and incubated overnight at 37C in 5% CO 2. Cells were then cultured in media containing 10% pooled human serum (Innovative Research, Novi, MI) at 37C in 5% CO 2 for 1 h to activate the complement system...

(9-23-14)

TSynthesis, physicochemical characterization, and selfassembly of linear, dibranched, and miktoarm semifluorinated triphilic polymers

WB Tucker, AM McCoy, SM Fix, MF Stagg, MM Murphy - Journal of Polymer Science , 2014Pooled normal human serum was purchased from Innovative Research (Novi, MI).(8-22-14)

Encapsulation of adenovirus serotype 5 in anionic lecithin liposomes using a bead-based immunoprecipitation technique enhances transfection efficiency

N Mendez, V Herrera, L Zhang, F Hedjran, R Feuer - Biomaterials, 2014Serum-induced particle aggregation. Human serum (Innovative Research, Novi, MI) was incubated in 96-well plates at 37 C and 5% CO 2 for 1 h before use for decomplementation.

(8-1-14)

A comparison of drug-induced cardiotoxicity in rat embryos cultured in human serum or protein free media

HE Ritchie, CH Svensson, MF Nilsson, WS Webster - Journal of Pharmacological and, 2014vitamins, inorganic salts supplemented with 4500 mg/L, glucose. The pooled, normal human serum (Innovative Research, Novi, MI 48377) was heat inactivated (56 C for 30 minutes) and supplemented with glucose (3 mg/ml).(6-24-14)

Depressive symptoms and cognitive performance in older adults

H Shimada, H Park, H Makizako, T Doi, S Lee, T Suzuki - of Psychiatric Research, 2014Low and high concentration quality control pools were prepared by adding 10 or 100 ng to 5 ml portions of human serum (Innovative Research, Novi, MI), giving nominal concentrations of 2 and 20 ng/ml, respectively...

(6-7-14)

Naturally enveloped AAV vectors for shielding neutralizing antibodies and robust gene delivery in vivo

B Gyrgy, Z Fitzpatrick, MHW Crommentuijn, D Mu - Biomaterials, 2014In vitro neutralization assays were performed with 10-donor pooled normal human serum (Innovative Research, Novi, MI) or Gammagard S/D purified intravenous immunoglobulin (IVIg), (Baxter, Deerfield, IL, kindly provided by Dr. Luk H. Vandenberghe, Harvard Medical School...(3-29-14)

Determination of total thyroxine in human serum by hollow fiber liquid-phase microextraction and liquid chromatography-tandem mass spectrometry

S Yong, Y Chen, TK Lee, HK Lee - Talanta, 20142.3. Samples. Extraction method development was carried out on human serum IPLA-SER2 (Innovative Research, Novi, MI, USA) and human serum...

(1-17-2014)

Chemically-Modified DNA Aptamers Bind Interleukin-6 with High Affinity and Inhibit Signaling by Blocking its Interaction with Interleukin-6 Receptor

S Gupta, M Hirota, SM Waugh, I Murakami, T Suzuki... - Journal of Biological ..., 2014Serum Stability Assay - SOMAmer (0.5 M) was incubated with 90% human serum (Innovative Research, Novi, MI, IPLA-SER) in SB17T buffer containing 0.01% TWEEN-20. Samples were incubated at 37C and aliquots were drawn at various time points from 0-48 hours.(1-17-14)

Reduction of nonspecific protein adsorption on cantilever biosensors caused by transverse resonant mode vibration

BN Johnson, R Mutharasan - Analyst, 2014 and 30% hydrogen peroxide (H2O2) were from Fisher. NanoOrange dye was from Invitrogen (Carlsbad, CA). Human serum (HS) was from Innovative Research (Novi, MI). Polyurethane (MC, clear) was from Wassar Corporation (Auburn, WA). Lead zirconate titanate (PZT-5A)...

(1-13-14)

Immunoproteomically Identified GBAA_0345, Alkyl Hydroperoxide Reductase Subunit C is a Potential Target for Multivalent Anthrax Vaccine

YH Kim, K Kim, YR Kim, MK Choi, HK Kim, KJ Choi... - Proteomics, 2013immunoproteomic analyses. A pooled sample of normal human sera was purchased from Innovative Research Inc. (Peary Court, Novi, MI, USA). Sera from five patients with anthrax were also used in this study. These patients contracted anthrax during Korean outbreaks in(1-12-14)

Chemically-Modified DNA Aptamers Bind Interleukin-6 with High Affinity and Inhibit Signaling by Blocking its Interaction with Interleukin-6 Receptor

S Gupta, M Hirota, SM Waugh, I Murakami, T Suzuki... - Journal of Biological ..., 2014 Serum Stability Assay - SOMAmer (0.5 M) was incubated with 90% human serum (Innovative Research, Novi, MI, IPLA-SER) in SB17T buffer containing 0.01% TWEEN-20. Samples were incubated at 37C and aliquots were drawn at various time points from 0-48 hours.

(12-13-13)

Maximizing Exosome Colloidal Stability Following Electroporation

JL Hood, MJ Scott, SA Wickline - Analytical Biochemistry, 2013 For isolation of human serum exosomes, pooled human serum (Innovative Research Inc., Novi, MI) was diluted 1:10 in PBS or 50 mM trehalose PBS, subjected to centrifugation and supernatantscollected at 300 xg for 10 min, 2000 xg for 10 min, and 10,000 xg for 30 min.(11-25-13)

Outer membrane protein P5 is required for resistance of nontypeable Haemophilus influenzae to both the classical and alternative complement pathways.

CV Rosadini, S Ram, BJ Akerley - Infection and Immunity, 2013and incubated at 37C for 30min with or without pooled normal human serum (NHS) from 158 healthy anonymous donors age 18-65 (final concentrations specified in the figures) (Innovative Research, Novi, Michigan) and plated on sBHI agar for CFU enumeration

(9-13-13)

RNA aptasensor for rapid detection of natively folded type A botulinum neurotoxin

P Janardhanan, CM Mello, BR Singh, J Lou, JD Marks... - Talanta, 2013 Carlsbad, CA). Molecular biology grade dithiothreitol (DTT) was obtained from Promega (Madison, WI). Pooled normal human serum was purchased from Innovative Research (Novi, MI). 2.2. Biotinylated RNA aptamer synthesis(1-9-13)

Proteolysis Breaks Tolerance Toward Intact a345 (IV) Collagen, Eliciting Novel AntiGlomerular Basement Membrane Autoantibodies Specific for a345NC1 Hexamers

F Olaru, XP Wang, W Luo, L Ge, JH Miner, S Kleinau... - The Journal of Immunology, 2013Normal human sera (Innovative Research, Novi, MI) were used as negative controls. Immunoassays ELISA was performed in MaxiSorp plastic plates coated overnight with NC1 monomers (100 ng/well) or NC1 hexamers (300 ng/well) and then blocked with 1% BSA

(12-16-12)

Engineering Polypeptide Coatings to Augment Gene Transduction and in vivo Stability of Adenoviruses

ZK Jiang, SBS Koh, M Sato, IC Atanasov, M Johnson... - Journal of Controlled ..., 2012Human serum (pooled from 10 donors) was purchased from Innovative Research, Inc., Novi, MI. Ad or Ad-K60KP370 was applied to AML-12 cells in serum-free media or serum-free media containing blood clotting factor, Factor X (FX), at physiological concentration (8 g/ml)(2-22-12)

Serum and plasma brain-derived neurotrophic factor (BDNF) in abstinent alcoholics and social drinkers

C DSa, RJ Dileone, GM Anderson, R Sinha - Alcohol, 2012 - ElsevierLow and High concentration quality control (QC) pools were prepared by adding 10 ng or 100 ng to 5 ml portions of human serum (Innovative Research, Novi, MI, USA), giving nominal concentrations of 2 and 20 ng/ml, respectively

(2-3-12)

The TonB3 system in the human pathogen Vibrio vulnificus is under the control of the global regulators Lrp and CRP.

AF Alice... - Journal of Bacteriology, 2012 - Am Soc Microbiolat -80C. In some experiments commercial pooled normal human serum was used (Cat IPLA-SER, Innovative Research, Novi, MI) with similar results to those obtained from other source. Ferric ammonium citrate (200 g/ml) (FAC) was added to allow V(12-23-11)

Selective enrichment and sensitive detection of peptide and protein biomarkers in human serum using polymeric reverse micelles and MALDI-MS

N Rodthongkum, R Ramireddy, S Thayumanavan... - Analyst, 2012 - xlink.rsc.orgbiomarkers. Experimental section. Reagents. Pooled normal human serum was acquired from Innovative Research (Novi, MI). ProteoSeek HSA/IgG removal kit was obtained from Pierce Biotechnology (Rockford, IL). Bradykinin

(11-28-11)

ApoB-100-containing Lipoproteins Are Major Carriers of 3-Iodothyronamine in Circulation

G Roy, E Placzek, TS Scanlan - Journal of Biological Chemistry, 2012 - ASBMB...500 l of normal pooled serum (human and rat serum from Innovative Research, Novi, MI; mouse serum from Millipore, Billerica, MA) was incubated with tracer quantities of 125 IT 1 AM for 24 h at 4 C in the presence or absence of excess unlabeled T 1 AM (50 m). Bound...(10-29-11)

New neutralizing antibody epitopes in hepatitis C virus envelope glycoproteins are revealed by dissecting peptide recognition profiles

A Kachko, G Kochneva, G Sivolobova... - Vaccine, 2011 - Elsevier...counted as positive. Negative controls were represented by normal mouse serum (NMS); normal human serum (Innovative Research, Novi, MI) and chimpanzee sera obtained prior to immunization/challenge. Titres are expressed...

(10-3-11)

The use of strong anion-exchange (SAX) magnetic particles for the extraction of therapeutic siRNA and their analysis by liquid chromatography/mass spectrometry

G Ye... - Rapid Communications in Mass ..., 2011 - Wiley Online LibraryHuman serum used for the siRNA stability studies was obtained from Innovative Research (Novi, MI, USA). Strong anion-exchange (SAX) beads were purchased from Invitrogen and Bioclone Inc(7-8-11)

Encapsulation and Release of Amphotericin B from an ABC Triblock Fluorous Copolymer

JP Jee, A McCoy, S Mecozzi - Pharmaceutical research, 2011 - Springer1H,1H,12H,12H-Perfluoro-1,12-dodec- anediol was purchased from SynQuest Laboratories Inc. (Alachua, FL). Human serum was obtained from Innovative Research (Novi, MI, USA). All solvents were of ACS grade or higher and were purchased from Sigma-Aldrich Inc. (St...

(7-1-11)

Development of a Sensitive Bead-Based Assay for Enhanced Monoclonal Antibody Detection

ME Ruidaz, N Mendez, AB Sanchez... - MRS ..., 2011 - Cambridge Univ Pressof 100ul of 3% BSA solution, 10ul of PBS with a specific concentration of monoclonal alemtuzumab antibody as obtained from the UCSD cancer center pharmacy (Genzyme, Cambridge, MA), 10ul of human serum, purchased from Innovative Research (Novi, MI) and Valley(5-15-11)

Modulation of Thyroid Hormone Concentrations in Serum of Rats Coadministered with Perchlorate and Iodide-Deficient Diet

T Kunisue, JW Fisher, K Kannan - Archives of environmental ..., 2011 - Springer...The accuracy and precision of the method were evaluated by the use of human serum and rat serum purchased from Innovative Research (Novi, MI). A procedural recovery test was conducted by using human serum as a representative matrix.

(4-19-11)

Inhibition of the Growth of Plasmodium falciparum in Culture by Stearylamine-Phosphatidylcholine Liposomes

GM Hasan, N Garg, E Dogra, R Surolia... - ... Parasitology Research, 2011 - hindawi.comHuman O serum was obtained from Innovative Research, Inc., Peary Court, Novi, Mich, USA...(10-30-10)

Efficient hydrolysis of the chemical warfare nerve agent tabun by recombinant and purified human and rabbit serum paraoxonase 1

M Valiyaveettil, Y Alamneh, L Biggemann... - ... biophysical research ..., 2010Briefly, human or rabbit serum (Innovative Research Inc., Novi, MI) was mixed with Cibacron Blue 3GA...Protection against soman or VX poisoning by human butyrylcholinesterase in guinea pigs and cynomolgus monkeys. Chem. Biol. Interact., 157-158 (2005), pp. 205-210.

(10-7-10)

The effect of physiological concentrations of sex hormones, insulin, and glucagon on growth of breast and prostate cells supplemented with unmodified human serum

A Esfahani, CWC Kendall, B Bashyam... - In Vitro Cellular & ..., 2010 - SpringerThese were then diluted to the desired concentrations with serum-free medium. For the experiments, the media were supple- mented with 10% commercial human serum (pooled serum from male and females, Innovative Research Novi, MI)(9-22-10)

Staphylococcus aureus resistance on titanium coated with multivalent PEGylated-peptides

X Khoo, GA OToole, SA Nair, BD Snyder... - Biomaterials, 2010 - Elseviersaturated peptide coating. Beads were washed three times in TBS-T then transferred into 100% human serum (Innovative Research; Novi, MI) and kept at room temperature on an orbital shaker. After various serum exposure

(5-14-10)

Quantification of monosaccharides through multiple-reaction monitoring liquid chromatography/mass spectrometry using an aminopropyl column

LA Hammad, DZ Derryberry... - ... in Mass Spectrometry, 2010 - Wiley Online LibraryLouis, MO, USA). PNGase F (5000 units/mL) was obtained from Northstar BioProducts (East Falmouth, MA, USA), and human blood sera were obtained from Innovative Research (Novi, Michigan, USA). C18 and active charcoal(8-19-09)

Rapid, direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization

C Wu, DR Ifa, NE Manicke, RG Cooks - Analytical chemistry, 2009 - ACS Publications(Portsmouth, NH). The serum sample, pooled from 10 individuals, was purchased from Innovative Research Inc. (Novi, MI) and stored in -20 C until analysis. The rat brains were purchased from Pel-Freez Inc. (Rogers, AR)

(10-16-08)

An efficient rHSV-based complementation system for the production of multiple rAAV vector serotypes

W Kang, L Wang, H Harrell, J Liu, DL Thomas... - Gene therapy, 2008 - nature.comH 2 SO 4 and detected by reading absorbance 450 nm. Pooled human serum (Innovative Research, Novi, MI, USA) was used as the hAAT positive control quantified as l.5 mg ml -1 of a-1-antitrypsin. Top of page References