牛凝乳酶原基因在食品级乳酸乳球菌中的重组表达.pdf

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牛凝乳酶原基因在食品级乳酸乳球菌中的重组表达.pdf_人人文库网

2025-05-10

蚂蚁淘(ebiomall)是苏州蚂蚁淘生物科技有限公司旗下的生物医学科研用品跨境直采（平行进口）平台，自营B2B电商。客户在蚂蚁淘搜索全球产品信息，找到合适的产品后再蚂蚁淘下单，然后蚂蚁淘的海外办事处进行跨境采购、运输到中国口岸，最后由蚂蚁淘国内团队清关并发货给客户

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德国MoBiTec公司于1987年由Stephan Diekmann 教授创立，由于和Max-Planck协会（德国处于领先地位的研究中心）有着密切的关系，这使得本公司与很多具有创造性的科学家有着亲密的合作。另外，MoBiTec 拥有来自欧洲和北美的研究人员，包括法国的Pasteur 研究中心和荷兰的Vrije大学的科学家。MoBiTec公司能提供独一无二的高质量产品,从而简化实验设计，而留出更多更宝贵的时间用于实际的研究。产品包括：双杂交和单杂交系统——体内蛋白-蛋白和DNA-蛋白关系，噬菌体展示系统——筛选特殊的多肽配体和选择具有特异性结合特性的新的蛋白质，Lamda PS基因文库，用于克隆的多功能DNA载体，靶基因的表达与分析，重组蛋白质高效表达和纯化试剂盒，细胞转染试剂和细胞培养工具，各种固体和溶解状态的酶，基因组学和蛋白组学研究产品（PCR，核酸，蛋白质纯化分析），各种抗体、细胞因子、生长因子、重组蛋白质，高级荧光试剂和试剂盒（蛋白质标记试剂盒，标记抗体，细胞染色试剂，钙指示器），亲和层析仪，凝胶分析工具——凝胶柱和凝胶电泳附件。Bacillus megaterium Expression SystemBacillus subtilis Expression VectorspBacTag Tagging VectorspPICHOLI Shuttle Vector SystemBroad-Host-Range Vectors pBBR122 & pBHR1Fusion Protein Cloning System PheBoFusion Protein Cloning System pAXBRP Plasmid and Competent BRP CellspPEPTIDE Cloning VectorPromoter-Trap Vector pBBR RESOpORF-CLONE Vector SystemExontrap Cloning VectorPCR Cloning Vector p3TSuicide Vector pCorrectClonessDNA-Production and Expression in pMEXHis-tag Cloning Vector pEG-His1Multiple Cloning Site Vector pMCS5Your partner for unique Two- and One-Hybrid-Systems, Phagemid Display, Genomic Libraries, DNA Vector Systems, Cell Biology and Antibodies, DNA and Protein Tools, Fluorescence Technology, Affinity Chromatography, and Lab Supplies

about MoBiTecHistorynnovative Tools for Molecular & Cell Biology

MoBiTec GmbH is a privately held company that was founded by Prof. Dr. Stephan Diekmann in 1987. The close relation to the Max-Planck Society as well as the location in Goettingen, one of Germany’s leading research centers, facilitated close cooperation with some of Germany’s most inventive scientists. In addition, MoBiTec has always been operating extensively with scientists all over Europe and North America, including those from the Pasteur Institute in France and Vrije University in the Netherlands. By commercializing the brilliant ideas of many researchers, MoBiTec is able to offer a unique range of high quality products with simplified protocols making lab life easier and leaving more time for actual research.

Products include e. g. two- & one hybrid systems for in vivo detection of protein-protein or DNA-protein interactions, a phagemid display system for screening specific peptide ligands and selecting novel proteins with specific binding properties, convenient Lambda PS genomic libraries; versatile DNA vectors for cloning, expressing and analyzing target genes, kits for efficient expression and purification of recombinant proteins, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, many products for genomics and proteomics research (e. g. for PCR, nucleic acid & protein purification and analysis) , numerous antibodies, cytokines, growth factors and recombinant proteins, superior fluorescence reagents and kits (e. g. protein labeling kits, labeled antibodies, cell staining reagents, calcium indicators), affinity chromatography products, gel analysis tools as well as general laboratory equipment such as lab columns and gel electrophoresis accessories.

In parallel to its own product lines, MoBiTec distributes products from several international companies in Germany including fluorescent probes and research chemicals (Molecular Probes, AnaSpec, Teflabs), quantum dots (Evident Technologies), a variety of DNA purification kits and DNA polymerases (Edge Biosystems, TaKaRa), diagnostic tools (Biomerica, Cytocell), gel elecrophoresis accessories (Amresco), glycobiology products (Dextra Laboratories), high-quality biochip products (GenTel), cell transfection reagents (Mirus) as well as antibodies, proteins, substrates, fine chemicals and kits for molecular and cell biology - in particular virology, immunology, neurobiology, apoptosis, signal transduction, cell proliferation, cytotoxicity and cancer research (MBL, A.G. Scientific, TaKaRa, Amresco, ViroGen, AnaSpec).

MoBiTec offers a total service, beginning with the clear and full documentation of products extending to individual advice and technical service for customers. MoBiTec products are distributed worldwide - in Germany from our home office, in other countries by distributors. Moreover, MoBiTec is continuously looking for new products and marketable technologies for licensing and is therefore strongly interested in scientific collaborations in order to develop innovative product ideas.

成核剂，播种和定相试剂盒。

Polyvalan的Crystallophore N o 1-一种发光的complex络合物，是有效的核清洁剂，可提供明显的同构和异常定相能力。唯yi可以一次解决三个问题的化合物。

˚F orming：种植能够产生高质量的衍射数据的晶体

˚F inding：识别晶体滴快速，准确地

博士ASING：在F\"〜30层的电子对铽3+大号III边缘在1.6或7.5千电子伏Naomi的Nucleants -Naomi的Nucleant促进了14种蛋白质的结晶，这是任何单个nucleant报道的zui高数量。这些蛋白质中的许多已被证明难以结晶，而其中一些蛋白质，包括膜蛋白质，仅在Naomi Nucleant存在的情况下才结晶。

微种子珠-微种子珠用于生成微种子-已\"粉碎”成碎片的微观晶体。

微晶播种的效果–IspD蛋白在微晶簇在液滴边缘成簇生长之前（左）和微晶种之后晶体分别生长（右）。（图片由Terese Bergfors提供）。

含有3个碘原子的分子排列在等边三角形中，可用于建立SAD和SIRAS实验的实验阶段。

产品编号产品名称单位大小单价数量

MD2-82 1号晶体电泳仪（小瓶，0.3mg） 0.3毫克MOLECULARDIMENSIONS1

MD2-80 1号晶体电泳仪（大瓶，0.6mg） 0.6毫克MOLECULARDIMENSIONS1

MD2-81 1号晶体荧光体（5个大的0.6mg小瓶） 5 x 0.6毫克MOLECULARDIMENSIONS 1

MD2-11 I3C相位套件 1套MOLECULARDIMENSIONS1

MD2-07娜奥米的努克利特 1个MOLECULARDIMENSIONS 1

MD2-14微种子珠（2.381毫米） 1 x 24珠MOLECULARDIMENSIONS 1

MD9-25细镊子 1个MOLECULARDIMENSIONS 1

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性能参数

产品名称：卓越性能，Mobitec巨大芽孢杆菌表达系统英文名称：Bacillus megaterium Expression Systems产品型号：kit产品规格：kit产品产地：德国品牌商标：MOBITEC价格：面议元

产品详细描述

说明书( $\"\"$ bmeg_handbook.pdf)

Features:

Stable, high-yield protein expression inBacillus megaterium
Ideal for both small and large-scale protein production
Tightly regulated and efficiently inducible xylose operon (up to 350-fold)
No alkaline proteases activity even up to 5 hours after induction
No endotoxins observed
Versatile cloning (extended polylinker, additionalBsrG I site*)
Versatile production (intracellular or extracellular)
Versatile purification (native, 6xHis-tag, Strep-tag, Strep/6xHis double-tag)
Removeable tag versions (for TEV or Xa proteases) available $\"New!\"$
Compatible with allB. subtilisvectors

*BsrG Isite is not included in all vectors.

TheB. megateriumexpression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.

B. megateriumhas proven to be an excellent alternative host toE. colifor heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

High Protein YieldAs a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.

Versatile System with a Wide Range of VectorsMoBiTec provides a wide range of useful vectors for theB. megateriumsystem that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.

B. megateriumprotoplasts specifically optimized for transformationThe protoplasts supplied by MoBiTec are fromB. megateriumstrain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.