M1801 Promega试剂 T4连接酶规格：100U

M1801 Promega试剂 T4连接酶 产品描述：

说明

T4 DNA Ligase(T4 DNA 连接酶) 催化粘性末端或平末端两条DNA 链的相邻核苷酸的5`- 磷酸基和3`-OH 的连接。该酶也可催化RNA 与双链DNA 或RNA 的连接，但不能使单链核酸相连。

特点

提供高浓度产品：Cat.# M1794 包含500 单位10 20u/ l 的T4DNA Ligase。 灵活：可用于5`、3` 或平末端DNA 插入片段。 提供10 反应缓冲液：300mM Tris-HCl (pH 7.8,25℃ )，100mM MgCl2，100mM DTT 和 10mM ATP。 蓝/白克隆验证：Promega 的蓝/ 白克隆检测为用于克隆的酶提供了高水平的质量控制。

原厂资料：

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5 -phosphate and the 3 -hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.

The T4 DNA Ligase Buffer Pack includes 3 tubes of T4 DNA Ligase 10X Reaction Buffer. The composition of the 10X reaction buffer is 300mM Tris-HCl (pH 7.8 at 25 C), 100mM MgCl2, 100mM DTT and 10mM ATP.

Features - BenefitsAvailable at High Concentration: Cat.# M1794 contains 500 units of T4 DNA Ligase at 10 20u/ l.Flexible: Use with 5 , 3 or blunt-ended DNA inserts.Provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25 C), 100mM MgCl2, 100mM DTT and 10mM ATP.Blue/White Cloning Qualified: Promega s blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.Choose Your Configuration: Learn more about our custom options for this product at: www.promega。。ｃｏｍ/custom/ApplicationsJoining double-stranded DNA molecules with cohesive or blunt ends.

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