Description:

Short Description: In this assay the SAP present in serum sample reacts with the anti-SAP. antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-SAP. antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum SAP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAP in the test sample. The quantity of SAP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Additional Information:

Name Related Product Names Gene Symbol Gene Fullname Gene id Alias Symbols Protein Name Swissprot ID Species Reactivity Assay Info Sensitivity Kit Range Homology NCBI Acc Number Standard Range Sample Type Product Format Specimen/Volumes Swiss Prot Number Applications Reconstitution and Storage Reactivity Kit Size Kit Principle Kit Component Kit Duration Storage Lead Time Intended Use Additional Information ::

Mouse SAP (Serum Amyloid P) ELISA Kit

Mouse SAP (Serum Amyloid P) ELISA Kit; SAP; 9.5S alpha-1-glycoprotein PTX2Mouse SAP (Serum Amyloid P)SAP

APCS

serum amyloid P-component

20219

serum amyloid P, SAP

Serum amyloid P-component

P12246

Mouse

Assay Methodology: Quantitative Sandwich ELISA

1.845 ng/ml

6.25 ng/ml - 200 ng/ml

Mouse

NP_001630.1

Refer To Manual

Serum, plasma and other biological fluids

In Solution

Refer To Manual

P02743

ELISA

1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-SAP conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-MOUSE SAP ELISA MICRO PLATEAnti-Mouse SAP coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. MOUSE SAP CALIBRATORThe lyophilized Mouse SAP calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 1 hour.

Mouse

96 well

Genway Biotech SAP ELISA Kit (Mouse) (GWB-B2F5E0) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Serum amyloid P-component has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Serum amyloid P-component - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serum amyloid P-component captured in the well.

ComponentAmount Anti-Mouse SAP Microplate 96 Wells (12 x 8 Well Strips) 20X Diluent Concentrate 50 mL 20X Wash Solution 50 mL 100X Enzyme-Antibody Conjugate 150 uL Chromogen-Substrate Solution 12 mL Stop Solution 12 mL Mouse SAP Calibrator 1

130 Minutes

Refer to kit instructions for component storage

Domestic: within 1 week deliveryInternational: 1 week

Research Use Only

Intended Use: The SAP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAP in biological samples of mice.

Reagents: 1. DILUENT CONCENTRATE (Running Buffer)One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution.3. ENZYME-ANTIBODY CONJUGATE 100XOne vial containing 150 ul of affinity purified anti-Mouse SAP antibody conjugated with horseradish peroxidase in a stabilizing buffer.4. CHROMOGEN-SUBSTRATE SOLUTIONOne vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.5. STOP SOLUTIONOne vial containing 12 ml 0.3 M sulfuric acid.WARNING: Avoid contact with skin.6. ANTI-MOUSE SAP ELISA MICRO PLATETwelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Mouse SAP.7. MOUSE SAP CALIBRATOROne vial containing a lyophilized Mouse SAP calibrator.