NAD/NADH检测试剂盒(荧光)
Application•For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.Direct NAD/NADH Assays in 96-Well PlateNEW!!!Key Features•Sensitive and accurate. Detection limit of 0.02 μM and linearity up to 1 μM NAD+/NADH in 96-well plate assay.•Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.•High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Method•F530/585nmSamples•Cell, tissue extracts etc. (Direct NAD/NADH Assays in 96-Well PlateNEW!!!)Species•AllProcedure•10 minSize•100 testsDetection Limit•0.02 μMShelf Life•6 monthsMore Details•Pyridine nucleotidesplay an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. BioAssay Systems EnzyFluo™ NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.
产品订货信息:
EFND-100 NAD/NADH检测试剂盒(荧光) 100次检测 ¥3492
EFND-048 NAD/NADH检测试剂盒(荧光) 48次检测 ¥2247
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