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GelRed® Nucleic Acid Gel Stain | Biotium

  
  2024-04-29
  
Ultra sensitive and environmentally safe replacement for EtBr DNA/RNA gel stain.Precast or post-elecrophoresis stainingAgarose, polyacrylamide, DGGE, EMSA, PFGE, or formaldehyde gelsRestriction enzyme mappingPCR amplicon detectionSouthern/northern blottingDNA band purification, cloning, sequencingCOMET assayCapillary electrophoresisCsCl gradients GelRed® and GelGreen® Nucleic Acid Gel Stains My product was accidentally left out at room temperature or exposed to light. Is it ruined? Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining. View more FAQs What is the difference between GelRed® and GelGreen®? Which one should I choose? The main difference between GelRed® and GelGreen® is their fluorescence excitation and emission wavelengths. GelRed® has red fluorescence, similar to ethidium bromide. GelGreen® has green fluorescence, similar to SYBR® Green or SYBR® Safe. Both dyes are compatible with standard UV transilluminators. GelGreen® is also compatible with blue light transilluminators, which allow users to avoid exposing themselves and their DNA samples to ultraviolet radiation.GelRed® and GelGreen® have higher sensitivity for double stranded nucleic acids compared to single stranded nucleic acids, but GelRed® is more sensitive for staining single stranded nucleic acids than GelGreen®. GelRed® is about twice as sensitive for double stranded nucleic acids compared to single-stranded nucleic acids, and about five times more sensitive than GelGreen® for staining single stranded nucleic acids.For more information about these products, please visit our DNA stains technology page.View the GelRed® Product LineView the GelGreen® Product Line View more FAQs Why do you offer GelRed® and GelGreen® in DMSO or water? The water formulation is a newer and improved product compared to the stock in DMSO. We recommend using dyes in water to avoid the potential hazards of handling DMSO, which can be absorbed through the skin. We continue to offer dyes in DMSO because some users do not wish to alter their established laboratory protocols. Based on internal testing, both formulations perform similarly. View more FAQs Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes. Size Catalog # price Shipping, Handling & Tax (CA only) not included in price. Learn more Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.Safer than EtBr: non-mutagenic and non-hazardous for disposalMuch more sensitive than EtBr and SYBR® SafeStable at room temperature and microwavableSimple precast or post-electrophoresis gel staining, no destainingReplaces EtBr with no change to equipment or optical settingsCompatible with downstream gel purification, restriction digest, sequencing, and cloningGelRed®: A Superior Alternative to EtBrGelRed® is much more sensitive than EtBr, and can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining without destaining. GelRed® can also be used to stain polyacrylamide gels via post gel staining. GelRed® is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning. GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. For detailed protocols for use, please download the GelRed® Product Information Sheet. Also see our GelRed® and GelGreen® Frequently Asked Questions (FAQs).Non-Mutagenic and Safer for the EnvironmentA series of safety tests have confirmed that GelRed® is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, working strength GelRed® can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download the GelRed®/GelGreen® Safety Report.How Safe is Your Gel Stain?Many so-called \"safe” DNA dyes like SYBR® Safe, Midori Green, GreenSafe, SafeView , and RedSafe not only have low sensitivity, but also readily penetrate living cells to bind DNA, and some are cytotoxic. Unlike these dyes, GelRed® is cell membrane-impermeant, so it cannot enter living cells to interact with their DNA. See our Gel Stains Comparison Flyer or  Gel Stains Comparison White Paper for details.Choose the Right Stain for Your ApplicationGelRed® 3X in water (catalog no. 41001) is ready-to-use for post-electrophoresis gel staining, and is supplied in a 4L Cubitainer®. Biotium also offers GelRed® 10,000X in water (catalog no. 41003) and GelRed® 10,000X in DMSO (catalog no. 41002). GelRed® in water is a newer, safer formulation and our recommended format. We continue to offer GelRed® in DMSO for established users who do not wish to alter their protocols. We also offer GelRed® Agarose and GelRed® Prestain Plus 6X Loading Dye.Also see GelGreen® Nucleic Acid Gel Stain, a safer replacement for SYBR® gel stains, which is compatible with visible light excitation.For more information, view our DNA Gel Stains Technology Page.GelRed® and its uses are covered by granted and/or pending US and International patents. GelRed and EvaGreen are registered trademarks of Biotium, Inc. SafeView is a trademark of Applied Biological Materials; RedSafe is a trademark of iNtRON Biotechnology. SYBR is a registered trademark of Thermo Fisher Scientific. Cubitainer is a registered trademark of Hedwin Corporation. GelGreen® is a sensitive, non-mutagenic and environmentally safer green fluorescent DNA gel stain. Detailed View A DNA ladder consisting of 13 DNA fragments ranging in size from 250 bp to 10 kb, provided pre-diluted in 1X loading buffer so that it is ready to load. Detailed View A DNA ladder consisting of 11 DNA fragments ranging in size from 100 bp to 1500 bp, provided pre-diluted in 1X loading buffer so that it is ready to load. Detailed View A non-UV, blue LED lightbox with a light source and filters for optimal detection of nucleic acids and proteins labeled with green or red fluorescent dyes. Detailed View A blue nucleic acid gel stain to visualize dsDNA in agarose or polyacrylamide gels by the unaided eye. Detailed View Activated charcoal in a convenient bag for decontamination of used gel staining solutions containing ethidium bromide or other toxic dyes. Detailed View Biotium implements a Quality System, certified by QAS according to Standard QAS ISO 9001:2015. Certificate No. US3564

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