Cellagentech/Repro-PACT™/K7228-500/0.5mL of 1000x
Product Description
According to literatures1-4, the supplement of small molecule cocktail (Repro-PACT) in cell culture media will simplify the process and increase the productivity in generating iPS cells. This process can be easily scale-up simply by culturing more cells. Repro-PACT can be used on many cell types, including primary skin fibroblasts, peripheral blood mononuclear cells, endothelial cells, or keratinocytes, and can be used in combination with any of the widely used reprogramming gene expression systems (e.g. retro/lenti-viral, episomal, mRNA, or proteins) to enhance the reprogramming to iPS cells.
Technical information:
| Cocktail Formulation: 1000x DMSO stock solution | DMSO solution contains: 0.5 mM of PD0325901, 0.5 mM of A83-01, 3.0 mM of CHIR99021, and0.5 mM of Thiazovivin | |
| Purity: | >98% (for each compound) |
Shipping Condition: The product is shipped in a glass vial at ambient temperature. Storage: Store DMSO solution at -20oC. Usage: Dilute Repro-PACT at 1:1000 into your basic ES media to make Repro-PACT media.
Reference:
| 1. | Lin et al. (2009) A chemical platform for improve induction of human iPS cells. Nature Method 6, 805-808. |
| 2. | Kawamata et al. (2010). Generation of genetically modified rats from embryonic stem cells. PNAS 107, 14223-14228. |
| 3. | Zhu et al. (2010) Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell Stem Cell 7, 651-655. |
| 4. | Yu et al. (2011) Efficient feeder-free episomal reprogramming with small molecules. PLoS ONE 6(3): e17557. |
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