Enzolifesciences/[pSer<sup>78</sup>]HSP27 polyclonal antibody/ADI-SPA-523-D/50µg-蚂蚁淘商城

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Enzolifesciences/[pSer⁷⁸]HSP27 polyclonal antibody/ADI-SPA-523-D/50µg

2025-05-11

Product Specification:

Alternative Name:	HspB1, Heat shock protein 27

Host:	Rabbit

Immunogen:	Synthetic peptide corresponding to a portion of human HSP27 phosphorylated at Ser⁷⁸.

UniProt ID:	P04792

GenBank ID:	L39370

Species reactivity:	Human, RatBovine, Guinea pig, Monkey

Applications:	IHC, WB

Recommended Dilutions/Conditions:	Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~27kDa by Western blot.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Handling:	Avoid freeze/thaw cycles.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Hsp27 is one of the most common members of the highly conserved and ubiquitously expressed family of small heat shock proteins (sHsp), which also includes alphaB-crystallin. It is characterized by a conserved C-terminal alpha-crystallin domain consisting of two anti-parallel beta-sheets that promote oligomer formation required for its primary chaperone function as inhibitor of irreversible protein aggregation. Hsp27 oligomerization is modulated by post-translational phosphorylation of Hsp27 at three serine residues, Ser15, Ser78, and Ser82, by a variety of protein kinases including MAPKAPK-3, PKAc-alpha, p70 S6K, PKD I, and PKC-delta. Hsp27 has been shown to inhibit actin polymerization by binding of unphosphorylated Hsp27 monomers to actin intermediate filaments. Anti-apoptotic functions of Hsp27 have also been identified through interactions with DAXX7, activation of Akt, and inhibition of apoptosome formation. Evidence suggests altered expression of Hsp27 is implicated in the pathogenesis of breast, ovarian, and prostate cancer.