Enzolifesciences/Cyclin D1 monoclonal antibody (5D4)/ADI-KAM-CC200-E/100µg-蚂蚁淘商城

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Enzolifesciences/Cyclin D1 monoclonal antibody (5D4)/ADI-KAM-CC200-E/100µg

2025-05-11

Alternative Name:	CCND1

Clone:	5D4

Host:	Mouse

Isotype:	IgG2a

Immunogen:	Recombinant human cyclin D1.

UniProt ID:	P24385

Species reactivity:	Human, Mouse

Applications:	Flow Cytometry, ICC, IHC (PS), IP, WB

Recommended Dilutions/Conditions:	Flow Cytometry (10µg/ml final concentration)Immunohistochemistry (10µg/ml, frozen/paraffin)Immunoprecipitation (1-10µg/200µg cell extract from 5x10⁶ cells)Western Blot (1µg/ml, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Application Notes:	Detects a band of ~36kDa by Western blot.

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 50% glycerol.

Shipping:	Shipped on Blue Ice

Long Term Storage:	-20°C

Scientific Background:	Cyclins are a family of proteins which function as the regulatory subunits of cyclin/cyclin-dependent kinase (Cdk) holoenzymes, mediating entry into and progression through the cell cycle. Each cyclin is expressed in a temporal fashion during the cell cycle, partnering with a specific Cdk to activate its protein kinase activity. The most well defined mitotic cyclins are: cyclin D, which partners with cdks 4 and 6 during early G1 phase; cyclin E, which activates Cdk2 in the G1 to S phase transition; cyclin A, which utilizes Cdks 1 and 2 during progression through S phase and into G2; and cyclin B, which activates Cdk1 in M-phase promotion.