- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Key Benefits
- Detection of reduced Thiols in cells or tissue extracts.
- Sensitive Fluorescent Assay.
- Detection of reduced Thiol levels in apoptosis, metabolism and oxidative stress.
- Detection of reduced Thiols in Bacterial, fungal, plant cells or other samples.
- Fluorescent 96 well Plate Reader Readout (excitation: 488nm and emission at 515-530 nm).
Additional information
| Kit Size | 100 |
|---|
During the normal course of metabolism, oxygen is partly reduced as electrons leak out of the electron transport chain during respiration. These partially reduced oxygen species (ROS) can react with organic substances through non-catalytic means. Furthermore, ROS can be generated via endogenous enzyme systems like plasma NADPH oxidase, cytoplasmic xanthine oxidase and organelle sources e.g., cytochrome P-450. ROS have been implicated in regulating diverse cellular functions including proliferation, defense against pathogens, intra-cellular signaling, transcriptional activation and apoptosis. Elevation of ROS beyond the buffering capacity of the cell can lead to oxidative stress. Elevated ROS levels can lead to damage of DNA/RNA, proteins and lipids which may lead to apoptosis. Cells have developed several mechanisms to counter act elevated ROS levels such as a thiol reducing buffer composed of cellular thiol levels (glutathione and thioredoxion) for the maintenance of the reduction-oxidation (redox) state of the cell, and enzymes to remove ROS (catalase, superoxide dismutase and glutathione peroxidase) (1-2).
Figure 1. Detection of glutathione (GSH) utilizing the Fluoro Thiol kit.
Figure 2. Jurkat cells were incubated with 1 mM staurosporine (4) for 3 hours. After which thiol levels were quantified using Cell Technology’s Fluoro Thiol kit. The graph represents approximately 1 x 10 4 cells per reaction (n=3).
| Document Title |
| Fluoro Thiol Protocol |
| Fluoro Thiol Datasheet |
| msds.FluoroThiol |
| Reference |
| Gamaley IA and Klyubin IV (1999) Roles of reactive oxygen species: Signaling and regulation of cellular functions. Int Rev Cytol 188:203–238. |
| Nakamura H, Nakamura K and Yodoi J (1997) Redox regulation of cellular activa-tion. Annu Rev Immunol 15:351–369. |
| 2,4-Dinitrobenzenesulfonyl Fluoresceins as Fluorescent Alternatives to Ellman s Reagent in Thiol-Quantification Enzyme Assays*. Hatsuo Maeda,*Hiromi Matsuno,Mai Ushida, Kohei Katayama,Kanako Saeki,and Norio Itoh. Angew.Chem.Int.Ed.2005 ,44 ,2922 –2925 |
| Marchetti,P.,et al .,Redox regulation of apoptosis: impact of thiol oxidation status on mitochondrial function.Eur.J.Immunol.,27 ,289-296 (1997). |
| Part# | Reagent | Temperature |
| Part# 4021 | Dye, 1 Vial Dried | Store at -20C |
| Part# 3053 | Lysis Buffer, 25 mL | Store at 2-8C |
| Part# 7015 | GSH Standard, 3 Vials Dried | Store at 2-8C |
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你的第一部分,白细胞和中性粒细胞偏低,说明免疫力低,最近要多保养,不受凉,近期容易生病。不过数据也不是太低的,也不用过于担心,多吃多睡,过一段时间复查,可能会好转为正常。另外消炎药,止痛,退热药尽量不吃,这些药会加重白细胞下降的。
第二部分,最主要的红细胞和血红蛋白是正常的,总体看还是正常的。但是下面数据有点低,而且只低一点,问题不大,多吃点米饭,红枣,肉等,会好转的。
第三部分,血小板部分是正常的。
你这种检查单,最主要的是免疫力低下,注意多吃多睡,别受凉,少烦神少劳累,引起白细胞低的药物尽量不吃。
病情描述(发病时间、主要症状等):
体检血常规中血小板计数343.血小板比积0.39.
尿检中白细胞计数26.2.上皮细胞计数10.30
心电图下写窦性心律,p-r间期偏短
想得到怎样的帮助:
有没有什么大问题
目前想建立家系血标本的淋巴细胞的永生化细胞株,请问有哪些成熟的方法及protol,费用贵么?
如题,求助。做肝癌细胞侵袭、迁移实验,用哪种细胞株比较好?
单纯从你这个尿常规结果来看:你的上皮细胞数量在正常女性的尿液中算是正常的,镜检白细胞:15——20个/HP,它表示你的泌尿系统可能存在感染。但是,因为没有你的其他资料和其他检查结果,单凭一个结果不能说明太多问题。一般情况下不可能是肾的不好,请不用担心。
我的建议是:
1、去医院复查,但要注意留尿的时候先清洁外阴,留取中段尿。及时化验。
2、如果检查结果仍然有白细胞,可去妇科或泌尿科看医生,一般的感染的话吃点药马上就好了。
3、放松心情,注意个人卫生即可。
希望有大神赐教!谢谢!
指导意见:
毕竟阴道上皮是鳞状上皮,而输尿管,膀胱,尿道的上皮是柱状上皮,形态上是不一样的.所以您要是对您的结果出怀疑态度,首先:重新留尿,做一个复检(留尿时注意防止阴道分泌物污染);或者让值班医生分析一下是那种类型的上皮(鳞状还是柱状).所以不要担心这个结果.一般情况下污染的比较多.
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