- SynonymC2,Complement C2,Complement Component C2
- SourceHuman C2, His Tag (MALS verified) (CO2-H52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Leu 752 (Accession # P06681-1).Predicted N-terminus: Ala 21Request for sequence
- Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 83.0 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.

Human C2, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human C2, His Tag (Cat. No. CO2-H52H7) was more than 90% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg (QC tested).
- BackgroundC2 is a major histocompatibility complex class-III protein. Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase. The lectin (LP) and classical (CP) pathways are two of the three main activation cascades of the complement system. These pathways start with recognition of different pathogen- or danger-associated molecular patterns and include identical steps of proteolytic activation of complement component C4, formation of the C3 proconvertase C4b2, followed by cleavage of complement component C2 within C4b2 resulting in the C3 convertase C4b2a.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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如题,求助。做肝癌细胞侵袭、迁移实验,用哪种细胞株比较好?
病情描述(发病时间、主要症状等):
体检血常规中血小板计数343.血小板比积0.39.
尿检中白细胞计数26.2.上皮细胞计数10.30
心电图下写窦性心律,p-r间期偏短
想得到怎样的帮助:
有没有什么大问题
你的第一部分,白细胞和中性粒细胞偏低,说明免疫力低,最近要多保养,不受凉,近期容易生病。不过数据也不是太低的,也不用过于担心,多吃多睡,过一段时间复查,可能会好转为正常。另外消炎药,止痛,退热药尽量不吃,这些药会加重白细胞下降的。
第二部分,最主要的红细胞和血红蛋白是正常的,总体看还是正常的。但是下面数据有点低,而且只低一点,问题不大,多吃点米饭,红枣,肉等,会好转的。
第三部分,血小板部分是正常的。
你这种检查单,最主要的是免疫力低下,注意多吃多睡,别受凉,少烦神少劳累,引起白细胞低的药物尽量不吃。
G418浓度之前师兄已经摸索出来,这次筛选直接用师兄浓度。
1、6孔板转染后多长时间开始进行G418筛选?
2、G418筛选是在6孔板中继续筛选还是转到24/96孔?大皿?
3、G418浓度什么时候减量
4、?筛选过程中荧光观察一小部分克隆不错,如何挑选出来?
5、整个筛选过程大约需要多长时间?
lipo3000筛选
谢谢大家!
目前想建立家系血标本的淋巴细胞的永生化细胞株,请问有哪些成熟的方法及protol,费用贵么?
希望有大神赐教!谢谢!

