Description
Details
Volume: 3.0ml
Cap Design: Lip Seal design and Silicone Washer Seal
Tube Design: Self-Standing with graduations
Designed for storing cells, blood, serum and other biological fluids at temperatures as low as -196 °C, these sturdy polypropylene vials offer a high level of chemical resistance but should be used only in the gas phase of liquid nitrogen.
The cap features a long skirt for easy one-handed aseptic technique and an inside thread design that will not contribute to possible contamination. This cap also features an exclusive silicone washer fitted inside the cap to ensure a positive seal at any temperature, even the lowest of cryogenic temperatures.
The tubes are provided with a white marking area for sample identification and can be color coded by the use of a CAPINSERT (Series T312). Only the non skirted vials can be centrifuged, and up to 17,000g. 12.5x71mm
Sterilized by gamma radiation and packaged in unique tamperproof, resealable, safety-lock bags of 100. Autoclavable. Certified RNase-, DNase-, Pyrogen- and DNA-free

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推荐了解“主细胞库”“工作细胞库”这两个名词。
① 在构建载体时,目的基因直接整合到细胞染色体组上,最好不要通过先瞬转在筛选稳定细胞株的这种方法,因为转染效率没有保证
② 高表达载体的构建,哺乳动物表达量一直是它自身的缺点,最好根据高表达载体定向的驯化细胞,提高蛋白表达量
③ 细胞的选择,筛选稳定细胞株我们常用的细胞是CHO,中国仓鼠卵巢细胞,由于CHO具有诸多的优点因此适合用于筛选稳定细胞株,而HEK293细胞则常用于瞬时转染
④ 后期的筛选,双抗预防污染,筛选细胞的时候抗生素浓度一定要做预实验,而且转染的时候不能有抗生素,关于细胞转染 稳定细胞系构建的相关理论
先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
建立稳定细胞株,一般是根据不同基因载体中所含有的抗性标志选用相应的药物对靶...专注整体实验·服务生命科学已开展了数千个实验外包项目

