Whole bovine blood is collected into vessels containing trisodium citrate as an anticoagulant. The blood is centrifuged and the plasma removed and frozen. The amount of trisodium citrate used can be specified by the customer.
| Specification - Bovine Plasma Citrated (PLSC) | |
| Product | Bovine Plasma Citrated |
| Catalogue No. | PLSC |
| Source | Bovine blood from healthy animals which have been inspected ante and post mortem by Australian or New Zealand government veterinarians and passed as fit for human consumption. |
| Collection Method | Blood is collected into sanitary vessels containing a customer specified trisodium citrate solution, centrifuged under temperature controlled conditions and immediately frozen. |
| Description | Non sterile straw coloured frozen liquid. |
| Pack Size | 25 litre polyethylene and nylon laminate bag with fattori closure packed in an outer cardboard carton, or to customer specification. |
| Storage | - 20 degrees Centigrade |
| Test | Method | Specification |
| Bioburden | Heterotrophic Colony Count CFUs | ≤ 5000ml |
| Haemoglobin | UV/VIS Spectrophotometer | ≤ 30mg/dl |
Hazards Identification
- Harmful in contact with skin and if swallowed
- Keep container tightly closed
- Wear suitable protective clothing and gloves.
First Aid Measures
If swallowed, wash out mouth with water provided person is conscious. Call a physician immediately.
If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen.
In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. Call a physician.
In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure flushing by separating the eyelids with fingers. Call a physician.
Fire fighting Measures
Extinguishing Media
Water spray. Carbon dioxide, dry chemical powder, or appropriate foam.
Special Firefighting Procedures
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
Accidental Release
- Wear chemical safety goggles, rubber boots and heavy rubber gloves.
- Spilled material should be carefully wiped up or moistened with water and removed.
- Ventilate area and wash spill site after material pickup is complete.
Exposure Controls / Personal Protection
- Safety shower and eye bath
- Mechanical exhaust required
- Wash thoroughly after handling
- Compatible chemical-resistant gloves
- Wash contaminated clothing before reuse
- Avoid contact with eyes, skin and clothing
- Chemical safety goggles
- Keep tightly closed
- Store in a cool dry place
Physical and Chemical Properties
Liquid
Stability and Reactivity
Stable for a limited time at 2° - 8°C.
Disposal Considerations
Contact a licensed professional waste disposal service to dispose of this material.
Observe all federal, state and local environmental regulations
Note: The above information is believed to be correct, but shall be used as a guide only.
Disclaimer: For pharmaceutical use only.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
各位大神，本人实验室小白一枚，现在要保存乙肝病人的血清，以后用来做实验感染细胞。为了最大限度保持病毒的感染活力，应该怎么保存呢？直接-80度冷冻可以吗？需要加甘油吗？
前段时间有个同学因为细胞状态不好，培养基里有小黑点，怀疑是支原体污染。于是检测了一下，果真是的，就买了支原体清除剂。别说效果还真好，3天后细胞状态就好了。
既然细胞状态好了，就停用了，没想到一停用，细胞状态就又差了。把培养箱、细胞房都清理一遍还是不行，就问到了我，既然这些因素都排除了，最后只有一个可能了，刚买的Gibco的胎牛血清，本来心里还想，同学实验室真是土豪啊，我就问了价格，南美胎牛1500……澳州胎牛血清3000……，简直惊讶到了，现在Gibco的血清简直是一货难求，澳州胎牛血清已经炒到了8000+了。
就断定这个血清肯定是假的，用检测试剂盒检测了一下，果然是这个问题，假血清真是害人啊，这些无良奸商……由于支原体比较小，滤器过滤不掉，所以大家买血清的时候一定要先试用，检测一下支原体（现在好多大牌子都会有支原体检测报告）
这里来分享一下支原体检测方法：
1，PCR检测法。
2支原体检测跑胶图-20084539882.jpg
这个Primers是依据支原体基因组中高度保守的16SrRNA编码域而设计的，只需简单的PCR反应就可检测M.Arginini,M.Fermentans,M.Hyorhinis,M.Orale,M.Salivarium,M.Hominis,M.Pneumonia等常见的支原体。
2，DNA萤光染色法
利用萤光染剂（bisbenzimide,Hoechst33258）侦测支原体污染。此染剂会结合到DNA之Adenosine-Thymidine(A-T)rich区域，因为支原体之DNA中A-T含量占多数（55～80%），所以可将其染色而侦测。被支原体污染之细胞经染色后，在细胞核外与细胞周围可看到许多大小均一之萤光小点，即为支原体之DNA，证明有支原体之污染。
Cell+细胞卫士__MycoTestKit(支原体检测试剂盒)：
支原体：黑色的，好象多为多形，培养液一般会浑浊，原体感染，国内血清很多都没有做支原体阴性检测，而支原体是牛血清中最常见的微生物之一。而且它不能用过滤的办法除去。支原体感染细胞以后，细胞病变不很明显，只是慢慢死去。
MycoTestKit是利用降落聚合酶链式反应技术（TD-PCR）对支原体16SrRNA基因高度保守区域特异性片段进行扩增检测。该方法灵敏度高，特异性强，可用于各种生物材料（如细胞培养基、实验动物分泌物、动物血清等）支原体感染的检测。
Cell+细胞卫士__NocardTreatment(支原体清除剂)：
（1）推荐NocardTreatment的稀释比例为1:1000，例如：1mL培养基加入1μL的NocardTreatment；
（2）弃去旧的培养基，用PBS将细胞清洗干净，再加入新鲜的含有NocardTreatment的培养基，1天1次，连续处理3-6天。
本人科研小白，研究新生！
课题准备检测血清中骨桥蛋白（OPN）含量
现在计划是先将所有血标本离心取血清先冻存，待所有标本收集齐后再统一做
大概要收集2个月，约70个病例。
现在我的问题就是：1、离心后的血清，如何分装、冻存？（具体条件、流程、用什么管子冻存，是否需要买冻存液等）
2、冻存后如何复苏？（最好有具体流程，具体复苏条件，是否需要买什么复苏液、培养基什么的）
刚来读书，一下子拿到这个题目有点懵，网上搜了一些，比较杂，比较乱，没有底，想请教各位哥哥姐姐给我提供点帮助，先谢谢了！
谁的答案详细、真实分就给谁...(勿灌水，否则必究之)
本人做动物实验，每组设7只，需要测定小鼠的相关指标，初次接触有几点不明白
1、测定指标时，是否可以每组7只，每只测定一次，一共测定7次？
2、是否可以每组选择3只，每只测定一次，共3次？
3、是否可以每组选择3只，每只自身平行测定3次，共9次？
4、测定指标时，是否可以前3只鼠测定A指标，后4只鼠测定B指标吗？
请问1、2、3中的三个方法哪种方法是否具有可行性？4中的测定方法是否可以？
