Whole bovine blood is collected into vessels containing trisodium citrate as an anticoagulant. The blood is centrifuged and the plasma removed and frozen. The amount of trisodium citrate used can be specified by the customer.
| Specification - Bovine Plasma Citrated (PLSC) | |
| Product | Bovine Plasma Citrated |
| Catalogue No. | PLSC |
| Source | Bovine blood from healthy animals which have been inspected ante and post mortem by Australian or New Zealand government veterinarians and passed as fit for human consumption. |
| Collection Method | Blood is collected into sanitary vessels containing a customer specified trisodium citrate solution, centrifuged under temperature controlled conditions and immediately frozen. |
| Description | Non sterile straw coloured frozen liquid. |
| Pack Size | 25 litre polyethylene and nylon laminate bag with fattori closure packed in an outer cardboard carton, or to customer specification. |
| Storage | - 20 degrees Centigrade |
| Test | Method | Specification |
| Bioburden | Heterotrophic Colony Count CFUs | ≤ 5000ml |
| Haemoglobin | UV/VIS Spectrophotometer | ≤ 30mg/dl |
Hazards Identification
- Harmful in contact with skin and if swallowed
- Keep container tightly closed
- Wear suitable protective clothing and gloves.
First Aid Measures
If swallowed, wash out mouth with water provided person is conscious. Call a physician immediately.
If inhaled, remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen.
In case of skin contact, flush with copious amounts of water for at least 15 minutes. Remove contaminated clothing and shoes. Call a physician.
In case of contact with eyes, flush with copious amounts of water for at least 15 minutes. Assure flushing by separating the eyelids with fingers. Call a physician.
Fire fighting Measures
Extinguishing Media
Water spray. Carbon dioxide, dry chemical powder, or appropriate foam.
Special Firefighting Procedures
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
Accidental Release
- Wear chemical safety goggles, rubber boots and heavy rubber gloves.
- Spilled material should be carefully wiped up or moistened with water and removed.
- Ventilate area and wash spill site after material pickup is complete.
Exposure Controls / Personal Protection
- Safety shower and eye bath
- Mechanical exhaust required
- Wash thoroughly after handling
- Compatible chemical-resistant gloves
- Wash contaminated clothing before reuse
- Avoid contact with eyes, skin and clothing
- Chemical safety goggles
- Keep tightly closed
- Store in a cool dry place
Physical and Chemical Properties
Liquid
Stability and Reactivity
Stable for a limited time at 2° - 8°C.
Disposal Considerations
Contact a licensed professional waste disposal service to dispose of this material.
Observe all federal, state and local environmental regulations
Note: The above information is believed to be correct, but shall be used as a guide only.
Disclaimer: For pharmaceutical use only.
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谁的答案详细、真实分就给谁...(勿灌水，否则必究之)
想做临床的血标本的一些代谢组学研究，不知道应该用血浆还是血清，大家的说法好像也不太一致，请大神指导啊，最好有些参考文献，谢谢！
热门血清品牌：
1.Gibco
【特点】
已通过ISO9001注册认证
通过最高水平的USP无菌性测试，并经过三重0.1μm过滤
具有ISIA可追溯性认证，血清的生产符合最高的可追溯性标准
澳洲来源特别适用于要求低风险的研究应用
购买推荐：http://www.biomart.cn/infosupply/27094281.htm
2.Hyclone
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【特点】
Sciencell的每一批胎牛血清均经过严格的质控和测试，包括近300种人类原代细胞筛选。胎牛血清经无菌过滤，pH值在7左右，内毒素不超过0.625EU/ml，且无病毒成分。
购买推荐：http://www.biomart.cn/infosupply/8505015.htm
国产血清推荐：
1.杭州四季青
购买推荐：http://www.biomart.cn/infosupply/33827963.htm
2.金源康
【特点】
质控标准：采自健康新生牛，两次0.1um过滤，无支原体；产品质量符合《中国药典》2015版标准。
产品特点：低lgG，低内毒素，促细胞生长优良
应用范围：娇贵细胞培养，种细胞的继代等。
推荐购买：
以下产品限时买二送一
新生牛血清（超级）http://www.biomart.cn/infosupply/20221746.htm
胎牛血清（优极）http://www.biomart.cn/infosupply/20323300.htm
胎牛血清（超级）http://www.biomart.cn/infosupply/25018976.htm
3.CellMax
推荐购买：
限时暑期特惠
优级胎牛血清http://www.biomart.cn/infosupply/14975942.htm
标准胎牛血清http://www.biomart.cn/infosupply/14975944.htm
澳洲特级胎牛血清http://www.biomart.cn/infosupply/14975935.htm
血浆是由抗凝的血液中分离出来的液体,其中含有纤维蛋白原,若向血浆中加入Ca2+,血浆会发生再凝固,因此血浆中不含游离的Ca2+.血清是由凝固的血中分离出来的液体,其中已无纤维蛋白原,但含有游离的Ca2+,若向其中再加入Ca2+,血清也不会再凝固.此外,血浆与血清的另一个区别是：血清中少了很多的凝血因子,以及多了很多的凝血产物.
各位大神，本人实验室小白一枚，现在要保存乙肝病人的血清，以后用来做实验感染细胞。为了最大限度保持病毒的感染活力，应该怎么保存呢？直接-80度冷冻可以吗？需要加甘油吗？
血清的基本成分是水，水中溶有蛋白质、脂肪、糖、无机盐、维生素等营养成分，也溶有人体代谢产物。血清中有抗体，这是被称作免疫球蛋白的蛋白质。
你说的应该称抗毒血清。
因制作时是将蛇毒、病原菌产的毒等小量多次地注射到免子、马血管内，每日慢慢加大注射量，一定时间后，因该动物体内产生抗体，经检测，达到一定效价后，就可以抽血。血液分离血清后再经提纯，就成了抗毒血清。
如蛇、白喉、破伤风、狂犬病抗毒血清等都是如此制备，用来治疗相应毒素反应。
该生物制剂是异种血清，对人会有过敏反应，注射前均要做皮试。阴性可注射，阳性必需作脱敏疗法才行。
1)提供基本营养物质：氨基酸、维生素、无机物、脂类物质、核酸衍生物等，是细胞生长必须的物质。
2)提供结合蛋白：结合蛋白作用是携带重要地低分子量物质，如白蛋白携带维生素、脂肪、以及激素等，转铁蛋白携带铁。结合蛋白在细胞代谢过程中起重要作用。
3)提供激素和各种生长因子：胰岛素、肾上腺皮质激素(氢化可的松、地塞米松)、类固醇激素(雌二醇、睾酮、孕酮)等。生长因子如成纤维细胞生长因子、表皮生长因子、血小板生长因子等。
4)对培养中的细胞起到某些保护作用：有一些细胞，如内皮细胞、骨髓样细胞可以释放蛋白酶，血清中含有抗蛋白酶成分，起到中和作用。这种作用是偶然发现的，现在则有目的的使用血清来终止胰蛋白酶的消化作用。因为胰蛋白酶已经被广泛用于贴壁细胞的消化传代。血清蛋白形成了血清的粘度，可以保护细胞免受机械损伤，特别是在悬浮培养搅拌时，粘度起到重要作用。血清还含有一些微量元素和离子，他们在代谢解毒中起重要作用，如SeO3,硒等
5)提供促接触和伸展因子使细胞贴壁免受机械损伤。
6)血清中有抗体，这是被称作免疫球蛋白的蛋白质，血清可以抗病毒,增强抵抗力血清属于生物制剂。
