
Human CD257 (B cell activating factor, BAFF,TALL-1, Blys, THANK) and CD256 (APRIL, a proliferation inducing ligand) are both type II molecules belonging to the TNF superfamily (TNFSFL #13b and 13 respectively). They are expressed by non-B cells, and are down regulated by mitogenic stimulation(2). BAFF and APRIL bind to at least two receptors: CD267 (TACI, transmembrane activator and CAML-interactor) and CD269 (BCMA, B cell maturation antigen), both of which are restricted to B cells(3,4). Ligation of these receptors with recombinant BAFF dramatically increases IgM production by peripheral blood B cells(1). A third receptor CD268 (BAFFR) is specific for BAFF has also been described(5). BAFF and BAFFR knockout mice have a reduced numbers of mature B cells in the periphery, however TACI and BCMA knockouts do not share this phenotype, suggesting that BAFFR may the primary receptor for BAFF in mice(8,9,10). Cell surface BAFF can be proteolytically cleaved to form a soluble trimeric molecule(2). Levels of soluble BAFF correspond with levels of autoantibodies in Sjogren’s Syndrome(11). Clone ANC2H3 binds to recombinant BAFF in EIA and Flow Cytometry and blocks binding of recombinant human CD257(BAFF)trn-muCD8 (catalog #525-020) to receptors on Raji cells in Flow Cytometry.
Isotype: Murine IgG1 Kappa
Immunogen: Recombinant soluble human CD257(BAFF)
Specificity: Antibody ANC2H3 binds to CD257(BAFF) in EIA and blocks binding of recombinant CD257(BAFF)-muCD8 to receptors on Raji cell surface in Flow cytometry.
Functional Application: Antibody ANC2H3 blocks binding of Recombinant CD257(BAFF) to Raji cell surface.
References:
1) Schneider P., J. Tschopp, et al. J. Exp. Med. 1999, 189(11):1747-1756.
2) Shu, H.B., H. Johnson, W.H. Hui. J Leukoc Biol 1999, 65:680-683.
3) Marsters, S.A., A. Ashkenazi, et al. 2000, Curr Biol 10:785-788.
4) Xia, X., H. Hsu, et al. 2000, J Exp Med, 192(1): 137-143.
5) Thompson J.S., C. Ambrose, et al. Science 2001, 293: 2108-2111.
6) Roschke, V, T.S. Migone, et al. J Immunol. 2002, 169: 4314-4321.
7) MacLennan, C.M., C.G. Vinuesa, 2002, Immunity 17:235-238.
8) B. Schiemann, et al, (2001) Science 293: 2111-2114.
9) S.M. Harless,et al, (2001) Curr Biol 11: 1988-1989.
10) Mol Cell Biol (2001) 21: 4067-4074.
11) X. Mariette, et al, (2003) Ann Rhem Dis 62: 168-171.
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适应症下会标注“本品适用于敏感菌所致......”字样;
药理作用下会标注“本品属......类抗菌素”字样。
2.β-内酰胺类青霉素(阿莫西林、氨苄西林、羧苄西林) 头孢菌素、碳青霉烯类、头霉素、单内酰环类、β-内酰酶抑制剂、甲氧青霉素类
3.大环内酯类 红霉素、白霉素(吉他霉素)、罗红霉素、阿奇霉素、克拉霉素、乙酰螺旋霉素、麦迪霉素、交沙霉素等
4 酰胺醇类:包括氯霉素、甲砜霉素等
5 四环素类:包括四环素、土霉素(强力霉素)、金霉素等
6 林可酰胺类 克林霉素、林可霉素
7.糖肽类抗生素: 比如万古霉素、替考拉宁
8 抗真菌抗生素:如灰黄霉素、两性霉素B
9 抗肿瘤抗生素:如丝裂霉素、放线菌素D、博莱霉素、阿霉素等
10 具有免疫抑制作用的抗生素如环孢霉素
11 多磷类抗生素:如磷霉素

